IAPP、SP免疫反应阳性细胞在不同发育阶段家兔 - 第三军医大学学报.DOC

胰岛淀粉样多肽和P物质在不同发育阶段家兔直肠的表达及分布特点 洪 艳，梁文妹，韩 晶，胡 赟，夏白娟，李一欣 （550004贵阳贵阳医学院组织学与胚胎学教研室） [摘要] 目的 观察不同发育阶段家兔直肠内胰岛淀粉样多肽(slet amyloid polypeptide，IAPP)、P物质(substance P,SP)的表达及分布特点。方法 收集生后5、15、25、35、60及90 d家兔60只，分6组，取直肠组织用免疫组织化学SABC法和图像分析法对IAPP、SP免疫反应阳性细胞的定位和表达进行。结果 生后5 d家兔直肠内可见少量散在分布IAPP阳性细胞，多位于上皮；5 d IAPP阳性细胞的数量呈明显上升趋势，至60 d达到最多(P0.05)，90 d时减少(P0.05)，主要分布于固有层。图像分析测得IAPP阳性细胞的平均灰度值随家兔的生长发育逐渐降低60 d达最低(P0.05)。SP阳性细胞于生后15 d始出现，35d后细胞数量明显增多(P0.05)，60 d达最高(P0.05)。其平均灰度值也于35d开始下降(P0.05)，于60 d达最低水平(P0.05)。结论 IAPP、SP在直肠的表达随家兔的生长发育而逐渐变化，推测者参与调节生后发育期间家兔直肠消化功能的成熟过程。胰岛淀粉样多肽P物质直肠生后发育家兔Expression of islet amyloid polypeptide and substance P in rectum of rabbits during the postnatal development Hong Yan，Liang Wenmei?，Han Jing，Hu Yun Xia baijuan，Li Yixin（Department of Histology and Embryologyedical Sciences, Guiyang Medical College, Guiyang,Guizhou Province,550004,China）[Abstract] Objective To investigate the expression and distribution features of islet amyloid polypeptide (IAPP),substance P （SP）in rectum of rabbits during the postnatal development. Methods Sixty rabbits were equally divided into 6 groups of the 5th, 15th, 25th, 35th, 60th, and 90th days. The rectum tissue were collected, fixed in Bouin solution, embedded in routine paraffin and then made into serial sections. The expression of IAPP and SP were observed by immunohistochemical SABC staining and image analysis . Results In the 5 days group, a few IAPP positive-cells were scattered into the mucosa epithelium of rectum .Since the 35 days group , the number of IAPP positive-cells ascended and obviously reached a peak in the 60 days group(P 0.05), and descended from the 60 to 90 days group(P 0.05). The positive cells were mainly distributed in the connective tissue of tunica propria. The mean grey values of IAPP positive-cells were decreased gradually during the postnatal development period and reached the lowest in the 60 days group(P 0.05). However, SP positive-cells were first present in the rectum in the 15 days g