生姜醇提取物抗心肌缺血再灌注损伤的作用研究Δ.PDFVIP

生姜醇提取物抗心肌缺血再灌注损伤的作用研究Δ ＊ # 卢仁福 ，贾 科，侯朋远，吴庆琛 (重庆医科大学附属第一医院胸心外科，重庆市 400016） 中图分类号 R285；R654.2 文献标识码 A 文章编号 1001- 0408（2010）11- 0970- 03 摘 要 目的：研究生姜醇提取物抗心肌缺血再灌注损伤的作用。方法：将50只雌性SD 大鼠随机分成假手术、模型及生姜醇提 取物高、中、低剂量组。结扎大鼠心脏左冠状动脉前降支30min ，再灌注90min ，复制急性局部心肌缺血再灌注损伤模型。测定大 鼠心肌组织匀浆中丙二醛（MDA ）含量、超氧化物歧化酶(SOD)活性；同时应用透射电镜观察心肌细胞超微结构的改变。结果：与 假手术组比较，模型组大鼠心肌组织MDA 含量显著升高（P ＜0.01），SOD 活性显著降低（P ＜0.01）；与模型组比较，生姜醇提取物 高、中、低剂量组大鼠心肌组织MDA 含量显著降低（P ＜0.01），SOD 活性显著升高（P ＜0.01）。透射电镜下超微结构显示生姜醇提 取物高、中、低剂量组大鼠心肌细胞组织结构以及线粒体的损害显著减轻。结论：生姜醇提取物可能通过保护线粒体、减轻脂质 过氧化程度、增强清除氧自由基的能力而保护心肌细胞。 关键词 生姜醇提取物；缺血再灌注损伤；心肌保护 Effects of Ethanol Extract of Zingiber officinale against Myocardial Ischemia- reperfusion Injury in Rats LU Ren-fu, JA Ke, HOU Peng-yuan, WU Qing-chen (Dept. of Cardiothoracic Surgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China） ABSTRACT OBJECTIVE ：To study the effects ethanol extract of Zingiber officinale against myocardial ischemia- reperfusion injury in rats. METHODS ：50female SD rats were randomly divided into sham group, model group, ethanol extract of Z. officinale groups (low, medium, high- dose group). Acute regional myocardial ischemia- reperfusion injury model was induced with ligation of rat heart left anterior descending coronary artery for 30min and 90min of reperfusion. The content of malondialdehyde (MDA) and activity of superoxide dismutase (SOD) were measured while transmission electron microscopy (TEM) was applied to detect the changes in myocardial ultrastructure. RESULTS ：As compared with sham group, the content of MDA in model group was significantly increased but the activity of SOD was decreased (P ＜0.01). As compared with model group, the contents of MDA in ethanol extract of Z. officinale groups were increased significantly while the activity of SOD increased (P ＜0.01). Results of ultrastructure change determined by TEM sho