中医肝肺关系的相关性探究.pdfVIP

提 要 本实验采用烟熏法复制大鼠肺气虚模型，大鼠夹尾激怒法复制肝郁化火的动物模 型，通过动物的一般情况及行为学观察，采集大鼠肺组织、肝组织等通过对其相关指 标的测定及观察，探究中医肝肺相关性的研究，深化对中医经典理论的认识，为临床 实践提供理论依据，以便使该理论更好的指导临床实践。 本实验选取健康Wistar雄性大鼠42只，体重（200±20）g，随机分成3组，正 常对照组，肺气虚组，肝郁化火组，每组14只。采用烟熏法复制大鼠肺气虚模型， 大鼠夹尾激怒法复制肝郁化火的动物模型，将各组大鼠相应造模30天，观测大鼠的 一般情况、行为，30天后处死动物。采集肝、肺组织标本，观察各组大鼠肺组织、 肝组织光镜下的病理改变及肺组织、肝组织匀浆中GSH、MDA等指标的变化，免疫组 化法检测肺组织MCP-1、ICAM-1、CCR2的表达，电镜下观察肺气虚组大鼠肝组织超微 结构的改变，应用SPSS13.0软件进行统计学处理实验数据。 研究结果表明，中医肝、肺之间互相影响，肝郁化火组大鼠肺组织中GSH、MCP-1、 ICAM-1、CCR2较正常对照组均有改变，肺气虚组大鼠肝组织中GSH、MDA水平及电镜 下肝组织超微结构较正常组均有所变化。本实验从多层次进一步验证了中医肝肺两脏 存在密切的相关性，从一定程度上揭示了中医理论关于肝肺相关性的实质，为中医学 脏腑相关的经典理论提供了科学依据和研究思路。 关键词 中医肝、肺；肺气虚；烟熏；肝郁 The related research on the relationship between Chinese medicine hepatopulmonary Speciality:Medical Department Of TCM Author: Wang Xiao Tutor:Prof. Zhang Wei Abstract Purpose: In our experiment, we replicated rat model of qi deficiency and liver depression due to angering rat tail folder,through smoking. Through studying general situation and behavior, collecting rat lung tissue, liver tissue,to observe relevant measurement and observation,we explore the relevance of traditional Chinese medicine hepatopulmonary research, deepen the understanding of the classical theory of Chinese medicine, provide experimental basis for clinical practice in order to make the theory better guide clinical practice. Mothed: Our experiment select 42 Wistamale rats r, body weighting(200 ± 20)g, randomly divided into 3 groups, normal control group, lung qi deficiency group, fire due to liver stagnation group, each 14. we replicated rat model of qi deficiency and liver depression due to