枸杞子的提取工艺研究 李广雷.pdfVIP

· 方 药 制剂分析 Western Journal of Traditional Chinese Medicine,2015 Vol.28 No.8 枸杞子的提取工艺研究* 1 2 1 1 1 1 1△ 李广雷 ， ， ， ， ， ， 杨 薇 林青华 魏雅蕾 昶国平 刘博翾 张宏桂 1北京中医药大学中药学院，北京100102；2北京悦康药业集团有限公司 [摘 要]目的：优选枸杞多糖的最佳提取工艺条件。方法：以枸杞子中枸杞多糖含量和枸杞多糖干膏得率 为指标，用硫酸-苯酚显色分光光度法测定其含量，用正交实验法L9(34)对实验提取料液比(A)、提取时间(B)、 提取次数(C)3个因素进行考察，确定枸杞多糖的最佳提取工艺，并对最佳工艺进行验证结果：以枸杞中枸杞多。 糖含量和枸杞多糖干膏得率为指标，最佳提取工艺为A3B2C3结论：由于提取次数从2次增加到3次后多糖的。 综合评价指数增幅不是很大，考虑到成本的原因，确定最佳的工艺条件为A3B2C2，即料液比为1:20，回流时间为 1小时，提取次数为2次。 [关键词]枸杞子；多糖；提取工艺 ［中图分类号］R285.1［文献标识码］A ［文章编号］1004-6852(2015)08-0018-03 tudyonExtractionTechnologyof S GouQiZi LI Guanglei1, YANG Wei2, LIN Qinghua1, WEI Yalei1, CHANG Guoping1, LIU Boxuan1, ZHANG Honggui1△ 1 College of Chinese Medicine of Beijing University of Traditional Chinese Medicine, Beijing100102, China; 2 Youcare Pharmaceutical Group Company Limited AbstractObjective: To optimize the extraction technology of GouQiZi (Lycium barbarum L.) polysaccharide. Methods: By taking the contents of GouQiZi polysaccharide and dry extract yield of GouQi polysaccharide as the indexes, the contents were measured by sulphuric acid-phenol reagent spectrophotometry, the extraction ratio of material to liquid (A), extraction time (B) and extraction times (C) were investigated by orthogonal test method L9 (34) to confirm the optimum extraction technology of GouQi polysaccharide, and then to verify the optimal process. Results: With the contents of GouQi polysaccharide and dry extract yield of GouQi polysaccharide as the indexes, the optimum extraction technology was A3B2C3. Conclusion: Since the extraction