丹酚酸a对心力衰竭大鼠心肌凋亡及心室重构的保护作用 - 南京医科大学.docVIP

丹酚酸A对压力超负荷大鼠心功能减退和心室重构的改善作用 丁 超，谢利平2，李荣成1* （1安徽省六安市市立医院心内科，安徽 六安 237000；2南京医科大学病理生理学系，江苏 南京 210029） [摘 要]目的：探讨丹酚酸A（Sal A）对压力超负荷大鼠心功能减退和心室重构的改善作用。方法：采用主动脉缩窄法（TAC）制备压力超负荷大鼠模型，并连续给予5 mg/(kg?d)的Sal A灌胃处理，同时部分压力超负荷大鼠仅注射等体积的生理盐水（saline），假手术（Sham）仅行开胸处理而不行TAC，依据实验设计将大鼠分为4组：Sham-saline组、TAC-saline组、Sham-Sal A组和TAC-Sal A组。分析12周后4组的血流动力学、心功能、心脏解剖学指标；采用苏木素-伊红（HE）、Massons三重及特异性荧光探针DHE染色分析心肌细胞的形态学、纤维化及活性氧自由基水平；免疫印迹法检测心肌细胞凋亡相关基因的蛋白水平。结果：与Sham-saline组比较，TAC处理可导致心率、左室收缩期平均压力、左室内压最大上升速率左室舒张末期压力Sal A处理对Sham大鼠的各项指标无影响。结论：Sal A可改善压力超负荷大鼠的心功能减退及包括心肌肥大、纤维化及凋亡在内的心室重构，具有较好的保护作用。 [关键词] 丹酚酸A；心心肌心肌mproving effect of salvianolic acid A on pressure overload-induced cardiac dysfunction and ventricular remodeling in rats Ding Chao1，Xie Liping2，Li Rongcheng1* （1Department of Cardiology, Luan Municipal Hospital, Luan 237000, China;2Department of Pathophysiology, Nanjing Medical University, Nanjing 210029, China） [Abstract] Objective: To explore the improving effect of Salvianolic acid A (Sal A) on pressure overload-induced cardiac dysfunction and ventricular remodeling in rats. Methods: The pressure overload rat model was established by transverse aortic constriction (TAC). The Sal A was applied at the concentration of 5 mg/(kg?d) by gavage for continuous 12 weeks, and some rats with pressure overload were treated with the same volume of saline. According to the experimental design, the rats were assigned into 4 groups: Sham-saline, TAC-saline, Sham-Sal A and TAC-Sal A. Twelve weeks later, the hemodynamic conditions, heart function and heart anatomy indicators were investigated among 4 groups. Hematoxylin-eosin (HE), Massons triple staining and specific fluorescent probe DHE were used to analyze the morphology, fibrosis and reactive oxygen species levels of myocardial cell. The protein levels of apoptosis related genes were evaluated by Western blotting. Results: Compared with Sham-saline group, TAC operation can lead to a decrease of heart rate, mean left ventricular systolic pressure, left ven