一种薏苡抗真菌蛋白的制备及抑菌活性研究.PDFVIP

46 2012, Vol. 33, No. 05 食品科学 ※基础研究 李 晨，白承之，李玉英，王转花* (化学生物学与分子工程教育部重点实验室，山西大学生物技术研究所，山西 太原 030006) 以薏苡(Coix chinensis)种子为实验材料，通过脱脂、水提、Resource S 阳离子交换层析和Superdex 75 凝胶 排阻层析等步骤纯化得到一种新的抗真菌蛋白，并对其抑菌活性进行初步鉴定。SDS分析显示该蛋白的分子 质量约为 28kD 。抑菌活性鉴定表明，该蛋白对链格孢霉(Alternaria alternate ) 、绿色木霉(Trichoderma reesei)和白 腐菌(Panus conchatus) 3 株丝状真菌具有显著的生长抑制活性，并呈现出剂量依赖的特征。本研究建立分离薏苡28kD 抗真菌蛋白的方法，制备方法简单易行，所得产品纯度达到 95% 以上。 薏苡种子；抗真菌蛋白；纯化 Isolation and Antifungal Properties of a New Protein from Adlay Seeds (Coix chinensis) LI Chen ，BAI Cheng-zhi ，LI Yu-ying ，WANG Zhuan-hua* (Key Laboratory of Chemical Biology and Molecular Engineering, Ministry Education, Institute of Biotechnology, Shanxi University, Taiyuan 030006, China) Abstract ：A protein from adlay (Coix chinensis) seeds was isolated by degrease with acetone and ether, extraction with 20 mmol/L Tris-HCl buffer, ion exchange chromatography on Resource S column and size exclusion chromatography on Superdex 75 column. The purity was higher than 95%. Partial characterization and antifungal activity were assayed. The protein exhibited a molecule mass of 28 kD in sodium dodecyl sulfate-polyacrylamide gel electrophoresis. This protein could exert potent inhibi- tory activity toward fungal species including Alternaria alternate , Trichoderma reesei and Panus conchatus in a dose-dependent pattern. Antifungal protein has become a popular research topic because of their resistance to pathogenic diseases. In this paper, the studies have demonstrated that adlay seeds have potentially exploitable significance and explored an effective method for the isolation and purification of antifungal proteins from plant seeds. Key words ：adlay seeds ；antifungal protein ；puri