比色法测定不同产地马齿苋中总多酚.PDF

现代药物与临床 Drugs Clinic 第 29 卷 第 7 期 2014 年 7 月 ·745 · 比色法测定不同产地马齿苋中总多酚 1 1, 2 1 1* 焦泽沼 ，刘佃雨 ，王培培 ，向 兰 1. 山东大学 药学院，山东 济南 250012 2. 成都市血液中心，四川 成都 610041 摘 要：目的 建立马齿苋药材中总多酚的测定方法，考察不同产地马齿苋药材中总多酚，为马齿苋药材质量评价提供参考。 方法 以没食子酸为对照品，采用福林酚（FC ）比色法测定马齿苋中总多酚。结果 不同产地的马齿苋药材中多酚含量在 6.30～17.37 mg/g。结论 FC 比色法简单快速，稳定，结果准确，适用于马齿苋中总多酚的测定；不同产地马齿苋中总多酚 差异较大，建议马齿苋药材中总多酚质量分数以没食子酸计不低于 6 mg/g 。 关键词：马齿苋；多酚；福林酚比色法；质量评价 中图分类号：R286.02 文献标志码：A 文章编号：1674 - 5515(2014)07 - 0745 - 04 DOI: 10.7501/j.issn.1674-5515.2014.07.011 Determination of total polyphenols in Portulaca oleracea from various habitats by colorimetric method 1 1, 2 1 1 JIAO Ze-zhao , LIU Dian-yu , WANG Pei-pei , XIANG Lan 1. School of Pharmaceutical Sciences, Shandong University, Jinan 250012, China 2. Chengdu Blood Center, Chengdu 610041, China Abstract: Objective To establish a method for determination of total polyphenols in Portulaca oleracea, to investigate the total polyphenols in this herb from various habitats, and to provide reference for quality evaluation of P . oleracea. Methods The total polyphenols content in P. oleracea was determined by Folin-Ciocalteu (FC) colorimetric method with gallic acid as the standard. Results The total polyphenols of 11 batches of P . oleracea varied from 6.30 to 17.37 mg/g. Conclusion FC colorimetric method is a quick, easy-operating, precise, and stable method, which is suitable for the determination of total polyphenols in P . oleracea. In consideration of big differences of total polyphenols among P . oleracea from various habitats, it is suggested that the total polyphenols in P . oleracea crude drug should be above 6 mg/g. Key words: Portulaca oleracea L.; polyphenols; Folin-Ciocalteu colorimetry; quality evaluation 马齿苋Port