多层海藻酸-壳聚糖聚电解质膜微球的制备与体外释放特性 - 中国药房.pdf

·制剂与工艺· 多层海藻酸-壳聚糖聚电解质膜微球的制备与体外释放特性研究 ＊ # 南艳微 ，郑晓玲（浙江大学医学院附属妇产科医院，杭州 310006） 中图分类号 R944 文献标志码 A 文章编号 1001-0408（2013）17-1581-03 DOI 10.6039/j.issn.1001-0408.2013.17.17 摘 要 目的：制备牛血清白蛋白-海藻酸-壳聚糖微球（BSA-ACM ），牛血清白蛋白-海藻酸-壳聚糖-海藻酸钠微球（BSA-ACAM ）， 牛血清白蛋白-海藻酸-壳聚糖-海藻酸-壳聚糖-海藻酸钠微球（BSA-ACACAM ）。方法：以海藻酸钠和壳聚糖溶液为囊材，对BSA 进行反复包裹，采用乳化-交联法制备BSA-ACM 、BSA-ACAM 、BSA-ACACAM ；采用扫描电镜测定微球粒径，Micro-BCA 试剂盒 测定载药量，考察包封率和24h 体外释药特性，并进行Higuchi 方程拟合。结果：BSA-ACM 、BSA-ACAM 、BSA-ACACAM 微球球 形圆整，分散性好，平均粒径分别为（3.79±1.33）、（3.52±0.96）、（3.07±1.17）μm ；载药量分别为（17.97±1.33）％、（16.95± 0.46）％、（16.47±1.49）％；包封率分别为（65.78±4.98）％、（63.99±4.83）％、（55.00±1.50）％。微球体外释放速率与聚电解质膜 包裹层数呈负相关，均符合Higuchi 方程（r 分别为0.9787、0.9869、0.9808），24h 内累积释放量分别为32.15％、25.59％、16.72％， 无明显突释现象。结论：多层海藻酸-壳聚糖聚电解质膜微球能减少药物的突释，具有良好的缓释效果。 关键词 牛血清白蛋白；海藻酸钠；壳聚糖；微球；制备；体外释放 Preparation and in vitro Release Study of Multilayer-sodium Alginate-chitosan Microspheres with Polyelectro- lytes Film NAN Yan-wei ，ZHENG Xiao-ling （Women ’s Hospital ，School of Medicine ，Zhejiang University ，Hangzhou 310006，China ） ABSTRACT OBJECTIVE ：To prepare Bovine serum albumin-alginic acid-chitosan microspheres （BSA-ACM ），BSA-alginic ac- id-chitosan-sodium alginate microspheres （BSA-ACAM ）and BSA-alginic acid-chitosan-alginic acid-chitosan-sodium alginate micro- spheres （BSA-ACACAM ）. METHODS ：With sodium alginate and chitosan as package material ，BSA was packaged repeatedly and then BSA-ACM ，BSA-ACAM and BSA-ACACAM were prepared with emulsion-crosslinking method. SEM was utilized to in- vestigate the particle size of the microspheres ，the drug-loading amount was detected by Micro-BCA kit ，and entrapment efficiency and 24h release behavior in vitro were also studied. The fitting of Higuchi equation was carried out. RESULTS ：BSA-ACM ，BSA- ACAM and BSA-ACACAM were spherical in shape and good in dispersibility. The mean diameters were （3.79±1.33）μm ，（3.52± 0.96）μm an