大鼠实验性蛛网膜下腔出血中fpn1和cp的表达研究 - 第三军医大学学报.doc

DOI：10.16016/j.1000-5404.201501160 大鼠实验性蛛网膜下腔出血后膜铁转运蛋白及铜蓝蛋白的表达 谭关平，刘 浏，何朝晖（400016重庆，重庆医科大学附属第一医院神经外科） [摘要] 目的 通过检测大鼠实验性蛛网膜下腔出血(subarachnoid hemorrhage, SAH)后脑组织内膜铁转运蛋白1（ferroportin 1，Fpn1）及铜蓝蛋白（ceruloplasmin，CP）铁代谢相关蛋白的表达，探讨铁代谢紊乱参与SAH后早期脑损伤（early brain injury,EBI）的机制。方法 96只成年雄性大鼠随机分为实验性蛛网膜下腔出血组（SAH组）和假手术组（sham组）。采用视交叉前池单次注血法构建大鼠SAH模型，SAH模型24h后进行取材。采用免疫组化、Western blot检测Fpn1及CP表达；应用伊文思蓝灌注法测定血脑屏障（blood-brain barrier,BBB）通透性改变；模型72h应用干湿质量法测定脑水肿比值。结果 与sham组相比，SAH组大鼠脑组织脑水肿及BBB通透性均有显著加剧（P0.05）；免疫组化及Western blot检测结果显示Fpn1及CP在大鼠大脑皮层区与海马区的表达均较sham组表达明显下调（P0.05）。结论 大鼠实验性SAH后铁代谢相关蛋白（Fpn1及CP）表达明显受抑制。 [关键词]蛛网膜下腔出血；铁代谢；脑水肿；血脑屏障通透性 Expression of Fpn1 and CP after Experimental Subarachnoid Hemorrhage in Rats Tan Guanping, Liu Liu, He Zhaohui. (Department of Neurosurgery, First Affiliate Hospital of Chongqing Medical University, Chongqing, China.) [Abstract] Objective To examine the expression of Fpn1 and CP in early period after experimental subarachnoid (SAH) in rats and discussion the iron metabolism-related proteins of Fpn1 and CP in SAH .Methods A total of 120 Male Sprague–Dawley rats were used. Rats were randomly divided into two groups: the SAH group and sham group. To construct the SAH rat model by using Injected the fresh arterial, non–heparinized blood into the prechiasmatic cistern (APC) induced subarachnoid hemorrhage. The animals were sacrificed 24h after SAH. Western blot and immunohistochemistry were used to examine the expression of Fpn1 and CP. Evans Blue perfusion was used to detect the BBB permeability. Wet and dry weight method was used to determine the ratio of brain water content 72h after SAH. Results Compared with the sham group, the brain water content of the rat brain tissue and the BBB permeability were significantly increased (p 0.05). Western blot and immunohistochemistry showed that, compared with the sham group ,the expression of Fpn1 and CP in rat cerebral cortex area was significantly downregulated (p 0.05). Conclusion The Fpn1 and CP expressions wer