桑黄菌发酵过程中酶活性的变化.pdfVIP

·420 · 中草药 Chinese Traditional and Herbal Drugs 第 45 卷 第 3 期 2014 年 2 月 桑黄菌发酵过程中酶活性的变化 * 祝子坪 ，李 娜 台州学院生命科学学院，浙江 台州 318000 摘 要：目的 研究桑黄菌发酵过程中酶活性的变化及其和胞外多糖量的关系。方法 在发酵过程中测定原始菌株和变异菌株 的羧甲基纤维素酶、滤纸酶、木质素过氧化物酶（LiP ）、锰过氧化物酶（MnP ）、漆酶（Lac ）活性变化及培养基中胞外多糖量 变化。结果 2 菌株的羧甲基纤维素酶和滤纸酶的活性变化呈单峰曲线，分别在第 4 天和第 6 天达到峰值；原始菌株和变异菌 株的 LiP、锰过氧化物酶和漆酶活性的变化呈双峰曲线，且原始菌株和变异菌株LiP 的酶促反应米氏常数（Km ）分别为25.96、 27.07 μg/mL ；MnP 的Km 分别为 13.28、13.49 mmol/mL；Lac 的Km 分别为 0.12、0.21 mmol/mL ；培养基中多糖量前期快速下 降，10 d 后趋于稳定。结论 桑黄菌发酵时能产生较全面的分解木质素和纤维素的酶系统，原始菌株和变异菌株产生的 LiP 和 MnP 为同种酶，Lac 为同功酶。桑黄菌胞外酶活性变化和培养基中胞外多糖量变化有一定关系。 关键词：桑黄菌；发酵过程；酶活性；羧甲基纤维素酶；滤纸酶；木质素过氧化物酶；锰过氧化物酶；漆酶 中图分类号：R282.15 文献标志码：A 文章编号：0253 - 2670(2014)03 - 0420 - 05 DOI: 10.7501/j.issn.0253-2670.2014.03.022 Changes of enzyme activity during fermentative process of Phellinus igniarius ZHU Zi-ping, LI Na School of Life Science, Taizhou University, Taizhou 318000, China Abstract: Objective To study the changes of enzyme activity and its relationship with the content of extracellular polysaccharide during the fermentative process of Phellinus igniarius . Methods The enzyme activities of carboxymethyl cellulase (CMC), filter paper enzyme (FPE), lignin peroxidase (LiP), manganese peroxidase (MnP), laccase (Lac), and medium extracellular polysaccharide content during the fermentative process of the original strains and mutant strains were investigated. Results The results showed that the changes of CMC and FPE activities of two strains showed a single-peak curve, peaks of which appeared in days 4 and 6, respectively, but the changes of LiP, MnP, and Lac activities showed a double-peak curve, and the Michaelis constant Km values of Lip in the original strains and mutant strains were 25.96 and 27.07 μg/mL. At the same time, the Km values of MnP were 13.28 and 13.49 mmol/mL, and Km values of Lac were 0.12 and 0.21 mmol/mL, r