二甲双胍对2型糖尿病模型大鼠胰岛素抵抗的影响.doc.docVIP

二甲双胍对2型糖尿病模型大鼠胰岛素抵抗的影响 秦 凤1，李春亮2，张惠莉1 (1青海大学附属医院内分泌科，青海省西宁市 810001；2青海大学医学院，青海省西宁市 810016) 引用本文：秦凤，李春亮，张惠莉. 二甲双胍对2型糖尿病模型大鼠胰岛素抵抗的影响[J].中国组织工程研究，2017，21():1909-1914. DOI:10.3969/j.issn.2095-4344.2017.12.018 ORCID: 0000-0003-2043-9136(秦凤) 文章快速阅读： 文题释义： 胰岛素抵抗：是指各种原因使胰岛素促进葡萄糖摄取和利用的效率下降，机体代偿性的分泌过多胰岛素产生高胰岛素血症，以维持血糖的稳定。胰岛素抵抗易导致代谢综合征和2型糖尿病。 胰岛素受体：是一个四聚体，由两个α亚基和两个β亚基通过二硫键连接。两个α亚基位于细胞质膜的外侧，其上有胰岛素的结合位点；两个β亚基是跨膜蛋白，起信号转导作用。 通过4周高脂饮食结合腹腔注射链脲佐菌素(35 mg/kg)建立2型糖尿病大鼠模型。4周高脂饮食时，模型大鼠同时口服二甲双胍4周[400 mg/(kg?d)]，对照组大鼠正常饮食，腹腔未注射链脲佐菌素。然后利用半定量RT-PCR、组织病理学及生化检测等方法进行相关分析。 结果与结论：①二甲双胍通过恢复2型糖尿病大鼠正常血脂，改善胰岛素抵抗症状；②二甲双胍上调脂肪代谢相关胰岛素受体和基因乙酰辅酶A氧化酶, 肉毒碱棕榈酰转移1和过氧化物酶体增殖物激活受体α；③二甲双胍治疗下调胎球蛋白A和视黄醇结合蛋白，2型糖尿病大鼠中降低的脂滴包被蛋白也恢复正常④在胰腺内，二甲双胍处理诱导胰岛素分泌和胰腺β细胞再生⑤结果说明，二甲双胍通过调节胎球蛋白A、视黄醇结合蛋白4、脂滴包被蛋白和脂肪代谢相关基因，改善2型糖尿病引起的胰岛素抵抗。 Effect of metformin on the insulin resistance in a rat model of type 2 diabetes mellitus Qin Feng1, Li Chun-liang2, Zhang Hui-li1 (1Department of Endocrinology, Affiliated Hospital of Qinghai University, Xining 810001, Qinghai Province, China; 2Medical College of Qinghai University, Xining 810016, Qinghai Province, China) Abstract BACKGROUND: More than 10 kinds of lipid metabolism-related genes have been found, and endocrine dysfunction mediated by these genes is an important pathological basis for the occurrence and development of insulin resistance. OBJECTIVE: To investigate the effects of metformin on serum lipid profiles and the expression levels of various genes associated with insulin resistance, as well as the histopathological changes of the liver and pancreas in rats with type 2 diabetes melitus. METHODS: A type 2 diabetes mellitus rat model was established by feeding a high-fat diet to the rats for 4 weeks, combined with the intraperitoneal injection of streptozotocin (35 mg/kg). In the meanwhile, metformin was administered orally (400 mg/kg?d) (model group) or nothing (control group). Semi-quantitative RT-PCR, histopathological and biochemical examinations were then performed.