pET-20b(+)载体说明.docxVIP

pET-20b(+)编号载体名称北京华越洋生物VECT4160pET-20b(+)pET20b载体基本信息别名:pET20b, pet 20b质粒类型:大肠杆菌蛋白表达表达水平:高克隆方法:多克隆位点，限制性内切酶载体大小:3716bp5 测序引物:T75 测序引物序列:??5-TAATACGACTCACTATAGGG-3载体标签:N-pelB信号肽, C-His载体抗性:Ampicillin备注:Hosts: E.coli. Related vectors: pBR322.稳定性:瞬时表达 Transient组成型:组成型 Constitutive病毒/非病毒:非病毒pET20b载体质粒图谱和多克隆位点信息pET20b载体简介The pET-20b(+) vector (Cat. No. 69739-3) carries an N-terminal pelB signal sequence for potential periplasmic localization, plus optional C-terminal His?Tag? sequence. Unique sites are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circular map. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase is shown below. The f1 origin is oriented so that infection with helper phage will produce virions containing single-stranded DNA that corresponds to the coding strand. Therefore, single-stranded sequencing should be performed using the T7 terminator primer (Cat. No. 69337-3).pET20b载体序列ORIGIN 1 ATCCGGATAT AGTTCCTCCT TTCAGCAAAA AACCCCTCAA GACCCGTTTA GAGGCCCCAA 61 GGGGTTATGC TAGTTATTGC TCAGCGGTGG CAGCAGCCAA CTCAGCTTCC TTTCGGGCTT 121 TGTTAGCAGC CGGATCTCAG TGGTGGTGGT GGTGGTGCTC GAGTGCGGCC GCAAGCTTGT 181 CGACGGAGCT CGAATTCGGA TCCGAATTAA TTCCGATATC CATGGCCATC GCCGGCTGGG 241 CAGCGAGGAG CAGCAGACCA GCAGCAGCGG TCGGCAGCAG GTATTTCATA TGTATATCTC 301 CTTCTTAAAG TTAAACAAAA TTATTTCTAG AGGGAAACCG TTGTGGTCTC CCTATAGTGA 361 GTCGTATTAA TTTCGCGGGA TCGAGATCTC GGGCAGCGTT GGGTCCTGGC CACGGGTGCG 421 CATGATCGTG CTCCTGTCGT TGAGGACCCG GCTAGGCTGG CGGGGTTGCC TTACTGGTTA 481 GCAGAATGAA TCACCGATAC GCGAGCGAAC GTGAAGCGAC TGCTGCTGCA AAACGTCTGC 541 GACCTGAGCA ACAACATGAA TGGTCTTCGG TTTCCGTGTT TCGTAAAGTC TGGAAACGCG 601 GAAGTCAGCG CCCTGCACCA TTATGTTCCG GATCTGCATC GCAGGATGCT GCTGGCTACC 661 CTGTGGAACA CCTACATCTG TATTAACGAA GCGCTGGCAT TGACCCTGAG TGATTTTTCT 721 CTGGTCCCGC CGCATCCATA CCGCCAGTTG TTTACCCTCA CAACGTTCCA GTAACCGGGC 781 ATGTTCATCA TCAGTAACCC GTATCGTGAG CATCCTCTCT