b0化铝比色法测定中药总黄酮方法的探讨.pdfVIP

MEDICINEANDMATERIAMEDICARESEARCH2008VOL．19NO．1 时珍国医国药2008年第19卷第1期’LISHIZHEN 三氯化铝比色法测定中药总黄酮方法的探讨 马陶陶，张群林，李俊’，孟晓明，黄成，陈玉璞 (安徽医科大学药学院，安徽合肥230032) 摘要：目的探讨以芦丁作为对照品，采用三氯化铝(A1C1，)比色法测定中药总黄酮含量的专属性，并建立A1C13比色法 测定中药总黄酮含量的最优条件。方法通过对几类典型结构的黄酮类及多酚类化合物A1C1，显色前后的吸收峰(200 —600 um)进行比较，分析以芦丁为对照品的A1C1，比色法测定中药总黄酮的合理性。结果A1C1，显色后，部分黄酮类物 质在421 am处有较强吸收。结论采用AICl3比色法测定中药总黄酮的含量具有合理性，并建立了AICl3比色法测定中药 总黄酮含量的最优条件。 关键词：A1C1，比色法；总黄酮；芦丁 中图分类号：R248．2文献标识码：A 文章编号：1008-0805(2008)01-0054-01 DeterminationofTotal for Flavonoids AICI，Colorimetry MA Tao-tao，ZHANGQun—lin，LIJun’，MENGXiao-ming，HUANGCheng，CHEN Yu—pu Anhui of of Medical‰泌船渺，Hefei230032，China) (CollegePharmacology Todiscussthe of withrutinasreference forevaluationon Abstract：Objective A1C11 substance thecontent speciality colorimetry total and the conditionof of flavonoids Tomakeaseriesofflavonoidsand investigateoptimum A1C13colorimetry．Methods poly． with with andmeasuretheir curvesat of structureA1C11 the 200—600am．ResultsAtthewave． phenolstypical absorption range 421 showedthesimilar with of am．someflavonoid一1ike rutin．ConclusionItisreasonabletOdeter- 1ength compounds absorption withrutinasreference minethetotalflavonoids substanceandestablishthe conditionof byusingA1C13colorimetry optimum A1C1， colorimetry．