泽泻汤对代谢综合征大鼠治疗作用和机理的探讨.pdfVIP

提 要 目的：探讨泽泻汤对代谢综合征（MS ）大鼠的治疗作用及其分子机理。方法： 以高糖高脂饲料建立MS 大鼠（雌雄）模型，将模型大鼠分为 3 组，分别给予泽泻汤 （中药组）、西布曲明（西药组）和生理盐水（盐水组）灌胃，并继续高糖高脂饮食， 连续 4 周。观察大鼠体重相关指标、血糖、血脂、血清胰岛素、瘦素和血浆神经肽 Y （NPY ）含量。应用免疫组化法检测下丘脑 Ghrelin、TNF- α，NPY 及其 Y1 受体亚 型（NPYY1R ，Y1R ）和胰岛NPY 及 Y1R ，用 image-pro plus6.0 进行平均光密度分 析。结果：中药组大鼠体重、血糖、血脂（TG、TC、LDL-c ）、血清胰岛素和瘦素、 血浆 NPY 含量均显著降低；下丘脑 Ghrelin、NPY 及 Y1R 和胰岛 NPY 及 Y1R 平均 光密度值亦显著低于生理盐水对照组。结论：泽泻汤能抑制 MS 大鼠的体重增长，降 低血糖，调节脂代谢，其作用可能通过抑制下丘脑 Ghrelin 合成，下调下丘脑和胰岛 NPY 及 Y1R 的表达，从而调节摄食中枢兴奋性，改善胰岛B 细胞功能，纠正胰岛素 抵抗和瘦素抵抗而实现的。 关键词 泽泻汤；代谢综合征；Ghrelin ；NPY ；NPYY1R ； CURATIVE EFFECTS OF ZEXIETANG ON METABOLIC SYNDROME RATS AND ITS MECHANISM Speciality: Basic Research of Integrated TCM and Western Medicine Author: Wu Zhichun Tutor: Professor Wang Hao Abstract Objective To explore the therapeutic effects of Zexie Tang on Metabolic syndrome(MS) rats as well as to make a study on the molecular mechanism. Methods Rats models(males and females) of Metabolic Syndrome were established by high sugar fat diet, then they were randomly divided into 3 groups, including ZeXieTang decoction(TCM group), sibutramine (western medicine group) and saline group. After 4 weeks of oral administration and high sugar fat diet, the changes of body weight and associated indicators, the levels of fasting blood-glucose （FBG ）, lipids, fasting insulin, serum leptin and plasma NPY were examined respectively. Meanwhile, Ghrelin, TNF- α, NPY and Y1 receptor subtype (NPYY1R ，Y1R) in hypothalamus, NPY and Y1R in pancreatic islets were tested by immunohistochemistry. The density mean was analyzed by image-pro plus6.0. Results The body weight, levels of FBG, lipids, insulin, leptin and plasma NPY were reduced significantly in TCM group. The density mean of Ghrelin, NPY and Y1R