小鼠单卵裂球体外培养及染色体制备.PDF

41 生命科学研究 Vol. 4 No . 1 2 0 0 0 3 Life Science Research M ar . 2000 : 1007-7847(2000) 01-0031-04 a 李秀蓉, 陆长富, 范立青, 朱文斌, 卢光 ( , 4 10078) : 为了探索研究植入前胚胎染色 病( 包括平衡易位) 诊断的可行性方法, 作者进行了 单卵裂球 外培养、染色 制备及显带技术研究.在 B 培养基加血清进行培养的基础上, 比 2 较了在两种不同处理因素进行 外培养时小鼠单卵裂球增殖情况. B 培养基加血清再加输卵 2 管包埋进行培养, 其单卵裂球 外增殖率为 0% ( 87/ 174) , 单卵裂球染色 制备成功率为 27. 6% ; B 培养基加腹水过滤液进行培养, 其单卵裂球 外增殖率为33% ( 78/ 237) , 单卵裂 2 球染色 制备成功率为3% . : 单卵裂球; 染色 ; 植入前胚胎遗传学诊断 : 343. 2; 9 3: Q Q A Culture and Chromosome Preparation of Mouseps Single Bl astomere in vitro - , - , - , - , - LI Xiu rong LU Chang fu FAN Li qing ZHU Wen bin LU Guang xiu ( , , 410078, , ) T he L ab of Hum an Reproductive Engineering Human M edical University Changsha Hunan China Abstract: It is reported that the mouseps single blastomeres w ere cultured in v it ro, then . prepared their chromosome and chromosome banding T he aim of the w ork w as to ex- plore the reliable method on human preimplantation genetic diagnosis ( PGD) especially chromosome diseases including chromosome translocation. The single blastomeres de- veloped in v it ro under different culture conditions w ere researched T he blastomerespde-. velopment rate in v it ro w ith a small fragment of mouse oviduct is 0%( 87/ 174) , and 48 ; 33%( 78/ 237) , 8 of them w ith visualizin