植物生物技术在生物制药中的作用.ppt

Figure 4-1. Detection of the hIGF-1 protein in transgenic tobacco and rice plants via western blot analysis. Lane 1: E. coli-derived rthIGF-1 (7.5 kDa); Lane 2: E. coli-derived rthIGF-1 added to tobacco leaf extract from a NT; Lane 3: prestained SDSmolecular weight standards from BIO-RAD; Lane 4: leaf extract from a transgenic plant transformed with the expression construct that contained the maize ubiquitin 1 promoter, the hIGF-1 coding sequence and the Nos-TER sequence; Lane 5: leaf extract from a NT. Biological activity tests Human SH-SY5Y neuroblastoma cell culture experiments showed that the plant-derived hIGF-1 was biologically active, promoting cell proliferation and cell differentiation. Figure 4-2. Graph A: Determination of the biological activity of the rthIGF-1 protein derived from the leaves of transgenic tobacco plants. Figure 4-3. Graph B: Determination of the biological activity of the rthIGF-1 protein derived from the leaves of a pUIGF-1 transgenic rice plant. EB: extraction buffer; NT: non-transformed leaf extract; plant #9: leaf extract from transgenic rice plant transformed with the hIGF-1 expression construct, pUIGF-1. Figure 5. Plant-derived rthIGF-1 protein is functional and influences the cellular proliferation and differentiation of the SH-SY5Y neuroblastoma cells. Panel A: E. coli-derived rthIGF-1 standard, 10 ng/ml led to proliferation of cells; Panel B: NT extract at equivalent protein concentration did not lead to proliferation or differentiation of cells; Panel C: protein extract from transgenic rice plant #9 that contained the rthIGF-1, 10 ng/ml led to proliferation of cells; Panel D: protein extract from transgenic rice plant #9 that contained the rthIGF-1, 50 ng/ml led to differentiation but not proliferation of cells. 分子农业具有许多优势，利用分子农业生产药物蛋白是一个很有前途的新兴领域，方兴未艾，值得探索。传统的药物研发周期长、投入大、风险高，成功后的回报也大。利用分子农业进行药物蛋白的研发则相对简单一些，因为蛋白的功能与疗效是明确的。 * hIGF-1 hIGF-1 Human SH-SY5Y neuroblastoma cells Human SH-SY5Y neuroblastoma cells (0