正交实验法在香果树种质离体保存中的应用.pdfVIP

2009,38(3):27-30. Subtropical Plant Science 正交实验法在香果树种质离体保存中的应用 洪森荣，尹明华 （上饶师范学院生命科学系，江西 上饶 334001 ） 摘 要：以香果树带芽茎段为外植体，采用正交实验法研究活性炭、琼脂、封口材料和培养容器对香果树试 管苗离体保存的影响。结果表明，香果树带芽茎段用 200 ml 三角瓶，无菌培养容器封口膜封口，保存于 MS + KT 2.0 mg/L + 6-BA 2.0 mg/L + NAA 0.1 mg/L + 30 g/L 蔗糖 + 0.5 g/L 活性炭 + 7.5 g/L 琼脂培养基上6 个月后， 成活率可达 86.3%。离体保存的试管苗没有发生遗传变异。 关键词：香果树；正交实验法；种质；离体保存 Doi: 10.3969/j.issn.1009-7791.2009.03.007 中图分类号：Q943.1 文献标识码：A 文章编号：1009-7791(2009)03-0027-04 Study on in Vitro Conservation of the Emmenopterys henryi Germplasm by Orthogonal Experiments HONG Sen-rong, YIN Ming-hua (Department of Life Science, Shangrao Normal Unversity, Shangrao 334001, Jiangxi China) Abstract：The stem segments with buds of Emmenopterys henryi were used as explants for studying the effects of activated carbon, agar, sealing materials and culture vessels on the in vitro conservation by orthogonal experiments. The stem segments with buds were cultured in 200 ml sterile erlenmeyer flask which was sealed by sealing film and contained MS basic medium, 2.0 mg/L KT, 2.0 mg/L 6-BA, 0.1 mg/L NAA, 30 g/L sucrose, 0.5 g/L activated carbon, and 7.5 g/L agar for 6 months. The results showed that the survival rate was up to 86.3%. The genetic variation of plantlets in vitro conservation did not appear. Key words：Emmenopterys henryi ; orthogonal experiments; germplasm; in vitro conservation 香果树（Emmenopterys henryi ）属茜草科（Rubiaceae ）香果树属高大落叶乔木，为中国特有的单 种属植物，是研究茜草科系统发育、形态演化及中国植物地理区系的重要材料，也是国家二级重点保 护植物和林业部公布的国家珍贵树种[1] ，主要分布在江西、浙江、福建、安徽等地的亚热带中山或低 [2]