傅里叶红外光谱法测定硫酸酯化葛仙米多糖的取代度.pdfVIP

150 2013, Vol.34, No.04 1,2 1,2 3 3 1,2,* (1. 445000 2. 445000 3. 445000) - A /A A /A H /H 1261BL1 1418BL1 0.993 Determination of Degree of Substitution of Sulfated Nostoc sphaeroides Kützing Polysaccharide by FTIR Spectroscopy 1,2 1,2 3 3 1,2,* ZHU Yu-ting TIAN Rui YANG Jie YUAN Jie MO Kai-ju (1. School of Biological Science and Technology, Hubei University for Nationalities, Enshi 445000, China 2. Hubei Key Laboratory of Biological Resource Conservation and Utilization, Enshi 445000, China 3. The Affiliated Hospital of Medical School of Hubei Institute for Nationalities, Enshi 445000, China) Abstract Sulfated Nostoc sphaeroides Kützing polysaccharides with different degrees of substitution (DS) were used as reference standards for DS determination by a barium chloride-gelatin turbidimetric method. FTIR spectroscopy was used for instrumental determination of DS. The baseline absorbance was recorded. Different combinations of probe band, reference band and baseline were comparatively evaluated based on the average relative deviation of ratio of Aprobe/Areference to DS. H 1261BL1/H 1418BL1 was found to be optimal for DS calculation with a correlation coefficient of 0.993 in comparison with DS values determined by the traditional method. Key words: Nostoc sphaeroides Kützing polysaccharide sulfate Fourier transform infrared spectrometer degree of substitution O657