λ - 卡拉胶降解组分的体外免疫活性 - 食品科学.pdfVIP

232 2009, Vol. 30, No. 11 食品科学 ※营养卫生 λ-卡拉胶降解组分的体外免疫活性 魏元臣，周革非，李树福，王长海 * (烟台大学海洋学院，山东 烟台 264005) 摘 要：目的：研究微波法降解得到的不同分子量角叉菜 λ- 卡拉胶的免疫活性。方法：以离子交换纤维素色谱 DE52 柱对微波降解所得 λ- 卡拉胶PC2(product of carrageenan 2)进行分级；从实验动物体内获取免疫细胞，采取体 外与多糖共同培养的方式，通过淋巴细胞、巨噬细胞增殖实验，中性红吞噬实验以及 NO 释放实验评价其免疫活 性。结果：纯化得到三个硫酸基含量和分子量依次增大的均一多糖分级组分 CF1(carrageenan fraction 1) 、CF2 (carrageenan fraction 2) 、CF3 (carrageenan fraction 3) 。实验结果表明，CF1 、CF2 、CF3 组分均能显著的促进巨噬 细胞和 ConA 诱导的脾细胞增殖，并能增强巨噬细胞吞噬能力和NO 的释放。结论：在体外培养研究条件下，λ- 卡拉胶降解组分对正常小鼠的免疫能力有促进作用。 关键词：多糖； λ- 卡拉胶；降解组分；体外免疫活性 in vitro Immunomodulatory Properties of Degraded lambda-Carrageenan WEI Yuan-chen ，ZHOU Ge-fei ，LI Shu-fu ，WANG Chang-hai* (School of Ocean, Yantai University, Yantai 264005, China) Abstract ：The microwave degradation product, named as PC2, of lambda-carrageenan, was separated into three fractions by DEAE-cellulose DE-52 column chromatography. T-lymphocytes and macrophage obtained from healthy mice were cultured in the presence of the three polysaccharide fractions to evaluate their immunocompetence through cell proliferation, neutral red phagocytosis and nitric oxide(NO) release tests. The results indicated that the contents of sulfate and molecular weights of carrageenan fraction CF1, CF2 and CF3 increased in turn. All these fractions could significantly promote Con A caused spleen T-lymphocyte proliferation and also increase phagocytic activity of mouse peritoneal macrophage and NO release. In conclusion, the degraded lambda-carrageenan is able to enhance the immunity of healthy mice. Key words ：polysaccharide ；lambda-carrageenan ；degraded fractions ；immunocompetence in vitro 中图分类号：Q949.2 文献标识码：A 文章编号：1002-6