antimicrobial activity of black pepper (piper …（抗菌活性的黑胡椒粉(piperu2026）.pdf

Global Journal of Pharmacology 7 (1): 87-90, 2013 ISSN 1992-0075 © IDOSI Publications, 2013 DOI: 10.5829/idosi.gjp.20104 Antimicrobial Activity of Black Pepper (Piper nigrum L.) S.K. Shiva Rani, Neeti Saxena and Udaysree Department of Botany, Osmania University College for Women, Koti, Hyderabad, India Abstract: Black pepper (Piper nigrum L.) native of south India popularly known as” king of spices”. Pepper is mostly used in the curry recipes as masalas and also as ingredient in the prescriptions of folk medicine, Ayurveda and traditional medicinal systems. The spicy tang of pepper is due to the presence of piperamides which are the pungent bioactive alkaloids accumulate in the skin and seeds of the fruit. Among them piperine is the major chemical constituent responsible for the bitter taste of the black pepper. In the present study piperine was evaluated for its antimicrobial activity against Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa, Escherichia coli , Alternaria alternata, Aspergillus niger, Aspergillus flavus and Fusarium oxysporum. The antibacterial activity was measured by agar well diffusion method and antifungal activity by poisoned food technique. Piperine showed antimicrobial activity against all tested bacteria with zone of inhibition ranged from 8-18mm. maximum zone of inhibition was against Gram positive bacteria Staphylococcus aureus (18mm) and minimum against Gram negative bacteria Escherichia coli (8mm). Piperine showed maximum antifungal activity towards Fusarium oxysporum (14mm) and very least effect against Aspergillus niger (38mm). The results showed significant activity of p