Biosynthesis of cyclic β-(1,2)-glucans inRhizobium leguminosarum biovarsviciae, phaseoli andtrifolii：（生物合成的环β-(1,2)葡聚糖inRhizobium leguminosarum biovarsviciae,phaseoli andtrifolii）.pdfVIP

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Biosynthesis of cyclic β-(1,2)-glucans inRhizobium leguminosarum biovarsviciae, phaseoli andtrifolii：（生物合成的环β-(1,2)葡聚糖inRhizobium leguminosarum biovarsviciae,phaseoli andtrifolii）.pdf

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Arch Microbiol (1995) 164:452 9 Springer-Verlag 1995 Olga A. Castro 9A n g e l e s Z o r r e g u i e t a 9Carlos S e m i n o Luis Ielpi Biosynthesis of cyclic JM1,2)-glucans in Rhizobium leguminosarum biovars viciae, phaseoli and trifolii lnstituto de Investigaciones Bioqulmicas Fundaci6n Campomar, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires and CONICET, Avenida Patricias Argentinas 435, 1405 Buenos Aires, Argentina V o l u m e 163, no. 6, p. 458: Fig. 4 s h o u l d a p p e a r as s h o w n below. Fig.4 A-C Comparison of glucoprotein intermediates from different phaseoli 8002, 8-10 R. teguminosarum biovar tr(folii RS800, l l A. tume- preparations of Rhizobium leguminosarum biovars, R. meliloti, and Agro- faciens A348. The inner membrane preparations corresponding to lanes bacterium tumefaciens. Different preparations of inner membranes of all 2, 3, 6, 7, and 8 were prepared in the presence of PMSF. C Samples were strains were incubated with UDP-[14C]glucose and MgC12 for 20 rain. obtained as in B, but SDSwas performed with a longer electro- Protein samples were subjected to SDSas described in Materi- phoresis run and samples were precipitated with trichloroacetic acid. Ra- als and methods. A Proteins stained with Coomassie blue. B Radioactiv- dioactivity was detected as in B. Lanes: 1 R. meliloti 102F34, 2, 3 R. legu- ity detected by fluorography with salicylic acid. Lanes: 1 A. tumefaciens minosarum biovar trifolii RS800, 4, 5 R. leguminosarum biovarphaseoli ME3, 2 R. leguminosarum biovarphaseoli 8002, 3 R. leguminosarum bio- 8002, 6 R. leguminosarum biovar viciae 128C53. Numbers on the left in-