重组木聚糖酶生产低聚木糖实验研究.pdf

31 2 Vo.l 31No. 2 2011 4 Chem istry and Industry of Forest Products Apr. 2011 欧阳嘉, 刘 明, 李 鑫, 陈牧, 连之娜 (南京林业大学 化学工程学院, 江苏 南京 210037) : 研究了重组木聚 酶 C0602生产低聚木 的制备工艺, 考察了不同类型底物底物浓度 和酶用量对酶解效率和低聚木 产率的影响实验结果表明, 低聚木 生产原料以抽提木聚 为 宜桦木木聚 质量浓度 30 g/L, 重组木聚 酶用量 20 IU /g 条件下水解 4 h, 酶解率可达 OUYANG Jia 56. 05% ,低聚木 产率( C2~ C6)为 29. 76% , 产品平均聚合度为 2. 76724. 5 g/L碱抽提玉米芯木 聚 在酶用量 80U /g条件下水解 48 h, 酶解率 68. 33% , 低聚木 产率为 14. 88% 产品不含木 , 平均聚合度为 3. 45 利用重组木聚 酶生产低聚木 可以大幅度降低产品中的木 含量,显著提高低聚木 产品质量 : 木聚 酶;低聚木 ; 酶水解; 聚合度 : TQ351 : A : 0253 2417( 2011) 02 0037 06 Experimental Investigation of Xylooligosaccharides Production by RecombinantXylanase OUYANG Jia, LIU M ing, LIX in, CHEN Mu, LIAN Zhina (The College of Chem ical Engineering, N anjing Forestry Un iversity, Nanjing 210037, China) Abstract: Xylooligosaccharides (XOs) production using the recom binant xylanase C0602 w as stud ied. Effects of d ifferent sub strates, substrate concentration and enzym e dosage on the enzym atic hydrolysis and xylooligosaccharide yield were investigated. The results show ed that the extracted xylan was the su itab le substrate for xylooligosaccharides production. When 30 g/L b irch w ood xylanwas treatedw ith the dosage of 20 U /g xylanase forhydrolysis4 h, the hydrolysis yieldwas 56. 05% and the xylooligo saccharide yield (C2 C6) w as 29. 76% . The average degree of polym erization( D ) was 2. 767. When 24. 5 g/L xylan extrac P ted from corncobw as hydrolyzed by 80U /g xylanase for 48 h, the hydrolysis yield rea