奶粉中金黄色葡萄球菌的检测（Detection of Staphylococcus aureus in milk powder）.docVIP

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奶粉中金黄色葡萄球菌的检测（Detection of Staphylococcus aureus in milk powder）.doc

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奶粉中金黄色葡萄球菌的检测（Detection of Staphylococcus aureus in milk powder）

奶粉中金黄色葡萄球菌的检测（Detection of Staphylococcus aureus in milk powder） Detection of Staphylococcus aureus in milk powder I. [experimental steps] 1. sample collection and submission Take 10 packs of small package of milk powder, pay attention to the integrity of the whole package and packaging. 2. sample handling After wiping the bag mouth with 75% alcohol cotton ball, hand disinfection gloves, open the bag mouth with sterile knife, carry on the sampling of the 10 packages with the aseptic ladle, and pay attention to replace the new aseptic knife when each sample is taken. In the shop has large sterile sulfuric acid paper precision for analysis on the balance of 0.1g respectively weighed 10 samples of each 25g and in 10 with glass beads Triangle flask, the replacement of the sterile sulfuric acid paper for a new scale of each sample, with mark pen in the bottom of the flask is numbered 1~10, with sterilization a 1 200ml and 1 50ml respectively to measure 200ml sterilization cylinder and 25ml 225ml has added 1 triangular flask with sterile saline warm furnace (first add a little milk to tune into a paste, then add all milk powder, to avoid caking), shake evenly, make fully dissolved after 1:10 dilution. Operation No. 2~10 flask is the same as No. 1. Summary: this instrument reagent process takes 75% alcohol cotton balls in a bag, a pair of sterile gloves, sterile sterile knife ten, ten scoops, sterile 20 sulfuric acid paper, analytical balance accuracy of 0.1g a, 300ml a flask of glass beads ten, mark pen, 200ml sterilization cylinder a 50ml cylinder, a sterilization, sterile saline 10L, 1L beaker 2. 3. enrichment culture Use the electric 7.5%NaCl broth medium is heated to melt, sterile Petri dish 10 9cm diameter with mark pen in the bottom plate were numbered 1~10, medium 45ml melt with sterilized measure of a 50ml into the No. 1 Petri dish, draw with a 5ml Straw sterile diluent 5ml a burn bottle added 1 Petri dish, 2~10 number of samples in the same method made the corres