低浓度臭氧对小鼠生殖生理的影响-中国试验动物学报.DOC

低浓度84消毒液对小鼠生殖生理的影响 胡玉，田韵，周丽，周军，丁志刚 (中南大学湘雅三医院医学实验中心，湖南长沙 410013) 【摘要】 目的 观察低浓度84消毒液对小鼠生殖生理的影响。方法 将72只KM小鼠随机分为三组，即：0.5% 84消毒液组（A组）、2.0% 84消毒液组（B组）和生理盐水组（C组），每组24只，雌雄各半，各组每天1次灌胃给予相应药物，连续9周。阴道涂片镜检观察雌性小鼠的动情周期。9周后，观察雄性小鼠的精子畸形率；并用ELISA法测定小鼠血清中性激素的水平；并取睾丸(或卵巢)、心、肝、肺、肾进行组织学观察。结果 与C组比较，A和B组小鼠体重差异无显著性（P ＞0.05）；血清性激素水平差异无显著性（P ＞0.05）；雌性小鼠动情周期差异无显著性（P ＞0.05）；组织学观察未见异常；与C组比较，A组小鼠精子畸形率差异无显著性（P ＞0.05），B组小鼠精子畸形率显著增高（P =0.041＜isinfection solution on reproductive physiology of mice HU Yu，TIAN Yun，ZHOU Li，ZHOU Jun，DING Zhi-gang ( The Center of Experimental Medical Research in the Third Xiangya Hospital，Centre South University，Changsha 410013，China ) 【Abstract】 Objective To study the effect of low-level 84 disinfection solution on reproductive physiology of mice. Methods 72 KM mice were randomly divided into three groups : 0.5% 84 disinfection solution group (group A), 2.0% 84 disinfection solution group (group B) and saline solution group (group C). 12 males and 12 females in each group. Corresponding medicine was given once a day by intragastric gavage for 9 weeks. The estrous cycle of female mice was observed by exfoliative cytology of vagina. After 9 weeks, The sperm abnormality test was performed, Serum samples were taken for sex hormone test by ELISA, and the histological change of testis (or ovary), heart, liver, lung and kidney were observed. Results Compared with group C, No significant difference was observed in the weight variation and serum sex hormone level in group A and B (P ＞0.05). The difference of the estrous cycle of female mice was not significant (P ＞0.05). No histopathological abnormality was observed . Compared with group C, There was has no significant difference in the rate of sperm abnormality in group A (P ＞0.05), was significant increased in group B (P =0.041＜0.05). Conclusion 05% 84 disinfection solution has no significant effect on reproductive physiology of mice, The concentration of disinfection solution must be strictly controlled when u