Caco_2细胞模型及其在中药吸收转运研究中的应用_赵静.pdfVIP

第 15 卷第5 期 中国实验方剂学杂志 Vol. 15,No. 5 2009 年5 月 Chinese Journal of Experimental Traditional Medical Formulae Ma , 2009 Caco22 细胞模型及其在中药吸收 转运研究中的应用 , * ( , 100700) [] Caco22 细胞模型是一种筛选药物离体口服吸收特性的模型, 现已广泛应用于评价药物在小肠的吸收特性和转运 机理的研究。作者描述了Caco22 细胞的培养及其细胞单层模型如何建立和验证;探讨了Caco22 细胞用于药物吸收转运研究的 机制; 对其在中药被动转运、载体介导的主动转运、中药外排机制和吸收过程中药物相互作用等方面的应用进行了详细的阐 述; 并评价了该细胞用于中药吸收转运机制研究中的作用;最后对该细胞模型可能的应用发展前景提出了展望。 [] Caco22 细胞模型; 中药; 吸收转运 [] R28515 [] A [ ] 10052 9903(200905 Caco22 Cell Model an d Its Applicat ion in th e Absorp tion and Tr an spor tation Resear ch of Chin ese Mater ia Medica * ZHAO J ing , LIANG Ai2hua ( Institute of Chinese Materia Medica , China Academy of Chinese Medical Science, Beij ing 100700, China ) [Abstract ] The Caco22 cell model is an in vitro model for screening drug oral absorption characteristics. It has been used widel for evaluating drug absorption characteristics and transportation mechanism. The authors describ the Caco22 cells culture and how to establish and verif the cell monola er model.The mechanism of its application in the drug absorption and transportation stud is investigated.The applications for passive transportation of Chinese material medica, vector mediated active transportation, efflux mechanism and the drug2drug interaction in the absorption process are discussed in detail.The efficienc on the absorption and transportation mechanism research of Chinese material medica for the Caco22 cell is evaluated. Finall , the prospect of the cell