大孔吸附树脂分离纯化洋葱皮黄酮的研究.pdfVIP

※工艺技术 食品科学 2011, Vol. 32, No. 12 133 大孔吸附树脂分离纯化洋葱皮黄酮的研究 1 1, 2 1 1 徐怀德 ，陈 佳 * ，包 蓉 ，刘 坤 ，李 晋 ( 1.西北农林科技大学食品科学与工程学院，陕西杨凌 7 12 100 ；2 .西北农林科技大学林学院，陕西杨凌 7 12 100) 　　 摘 要：以黄酮含量为指标，比较6 种大孔吸附树脂对洋葱皮黄酮的吸附和解吸效果。通过静态吸附与解吸实验， 筛选出效果较好的X-5 树脂进行动态实验研究。结果表明：X-5 树脂纯化洋葱皮黄酮的工艺条件为吸附流速2mL/min 、 上样溶液p H 5.0 、上样液质量浓度0 .5mg/mL 、50mL 80% 乙醇作为洗脱液，洗脱流速lmL/min 。经X -5 树脂纯化 后，洋葱皮黄酮纯度从7 .9 5% 提高到80 .7 8% 。三次重结晶后其纯度可达94 .5% 。该方法简单可行，纯化效果好， 适合于工业化生产。 关键词：大孔吸附树脂；分离；纯化；洋葱皮；黄酮 Separation and Purification of Flavonoids from Onion Peel by Macroporous Resin Adsorption 1 1, 2 1 1 XU Hu ai-de ，CHEN Jia * ，BAO Rong ，LIU Kun ，LI Jin (1. College of Food Science and Engineering, Northwest AF University, Yangling 7 12 100, China ； 2. College of Forestry , Northwest AF University , Yangling 7 12 100, China) Abstract ：The characteristics of adsorption and desorption of onion peel flavonoids on six kinds of macroporous resins were compared. Static adsorption and desorption experiments indicated that X-5 resin was the best resin for the separation and purification of flavonoids from onion peel. The optimal process parameters for purifying onion peel flavonoids with X-5 resin were sample loading flow rate of 0.5 mg/mL, sample pH 5.0 and 50 mL of 80% ethanol of as eluent at a flow rate of 1.0 mL/min. After the purification, the purity of flavonoids from onion peel was increased from 7.95% to 80.78% and could reach up to 94 .5% after further triple recrystallization. The developed method is simple and feasible for indu strialization. Key words ：macroporou s adsorption resin ；separation