细胞培养文献.pdfVIP

Growth of mammalian cells at high oxygen concentrations A. R. OLLER* , C. W. BUSER , M . A. TYO and W. G . THILL Y Center for Environmental Health Sciences, Massachusetts Institute of Technology, Cambridge MA 02139, USA • Author for correspondence Summary Oxygen, although essential to the growth of mam- trations ranging from 60/iM to 410/iM. We conclude malian cells in vitro and in vivo, has been widely that exponential growth at nearly maximum growth reported to be toxic at concentrations at or above the rates was observed for Sp2/0-derived cells, TK6, oxygen concentration in culture medium equilib- and Vero cells at constant oxygen concentrations up rated with air (approximately 200 /iM). We were to 400/iM (twice air saturation). therefore surprised to note that a diploid human These findings have substantial implications for B-cell line (TK6) was able to proliferate normally process control in the production of cells and cell- while exposed to 380/iM-oxygen. This observation derived materials. The intrinsic oxygen transfer was extended to Vero (African Green monkey kid- rate of any cell culture vessel can be increased ney) cells, and Sp2/0-derived murine transfectomas severalfold by raising the oxygen gas concentration. producing antibody. Using an experimental system In practice, this should permit significant increases with a high capacity for oxygen transfer, we deter- in the operating cell density without the difficulty mined the growth rates of the three cell lines at