四氯联苯对鸡外源原始生殖细胞迁移的影响.PDF

中国环境科学 2010,30(4) ：553~556 China Environmental Science 四氯联苯对鸡外源原始生殖细胞迁移的影响 张易祥 1,金秀梅 2,李赞东 2*,刘 莉 1,采克俊 1,丁志丽 1 (1. 中国水产科学研究院湖州师范学院,细胞工程研究 所,浙江 湖州 313000 ；2. 中国农业大学生命科学院,北 100094) 摘要：为了研究环境污染物四氯联苯对移植的原始生殖细胞增殖性迁移的影响,将发育至 14 期的供体鸡胚原始生殖细胞用 PKH26 荧光染 料标记,然后移植到经过四氯联苯处理的受体鸡胚生殖新月区域,继续孵化,检测其迁移和增殖情况.结果表明,和对照组相比, 四氯联苯可明 显降低移植原始生殖细胞在血液和性腺中的数量;四氯联苯对外源性原始生殖细胞迁移有明显影响,但雌二醇对外源性原始生殖细胞的增 殖性迁移无明显影响,揭示四氯联苯可能影响的是受体性腺的诱引物质,且这种影响是非雌激素样的作用. 关键词：鸡；原始生殖细胞；四氯联苯 中图分类号：X171.5 文献标识码：A 文章编号：1000-6923(2010)04-0553-04 1 2 Effects of tetrachlorobiphenyl on the exogenous primordial germ cells in chick. ZHANG Yi-xiang , JIN Xiu-mei , LI Zan-dong 2* 1 1 1 , LIU Li , CAI Ke-jun , DING Zhi-li (1.Insititute of Cell Engineering, Huzhou Teachers College, Chinese Academy of Fishery Sciences, Huzhou 313000, China ；2.College of Biological Sciences, China Agricultural University, Beijing 100094, China). China Environmental Science, 2010,30(4)：553~556 Abstract ：To study the effects of tetrachlorobiphenyl on the migration of the primordial germ cells (PGCs) multiplication with utilizing poultry embryonic primordial germ cell migration features, the donor PGCs isolated from the chick embryos at stage 14 were labelled with PKH26. The labelled PGCs were transplanted into the germinal crescents of recipient embryos treated with tetrachlorobiphenyl, which were then incubated. The labelled donor PGCs were detected at stages 13~15 and stages 27~29. The number of the exogenous PGCs decreased significantly at stages 13~15 and stages 27~29 compared with controls (P0.01). Tetrachlorobiphenyl had significantly effect on the migration of exogenous PGCs, but estradiol had no effect. The result suggests that tetrachloro