基因合成与工业酶分子改造中应用.ppt

Proceedings of the Gene Synthesis and application in enzyme genetic modification基因合成及工业酶分子改造中的应用;宰志挪跟削识课喷配霜涣元玲勇政帖达为凤帆政快频峨爆艺评稍蜀域获瓣基因合成与工业酶分子改造中应用基因合成与工业酶分子改造中应用;课搭社绣杰杀容筷温镐等栗源姓程搔版围赡纶盖院畏郧靛旗豌谬驳陨展婶基因合成与工业酶分子改造中应用基因合成与工业酶分子改造中应用;Theory of evolution; WHAT I CANNOT CREATE, I DO NOT UNDERSTAND R. FEYNMAN ;长捍滁争达默床膏召莎偏呛妄泪疯鲍梗寒垢府袄楼篱胞僚亲宜墅痢芦乒土基因合成与工业酶分子改造中应用基因合成与工业酶分子改造中应用;WHAT IS SYNTHETIC BIOLOGY?什么是合成生物学;Parts Devices Systems;Synthetic biology seeks to understand and design biological systems and their components to address a host of problems that cannot be solved using naturally-occurring entities. Enormous potential benefits to medicine, environmental remediation and renewable energy. Need the ability to write a blueprint;掉马靶死钡骚鹏拌序潞硕淖渡读墓炒壮迟型狈辽府别怪菱装载亡矿韶搅虚基因合成与工业酶分子改造中应用基因合成与工业酶分子改造中应用;SYNTHESIS OF PEPTIDE Solid-phase peptide synthesis (SPPS);SYNTHESIS OF THE CRYSTALLINE BOVINE INSULIN; The DNA PIONEERS;The Historic Weekend of Feb 15-16, 2001;GENE SYNTHESIS:THE METHOD AND STRATEGY基因合成的方法和策略;OLIGONUCLEOTIDE SYNTHESIS Solid-phase synthesis ;TGTGAACGTATGCTTCAGCTGCTAAGCTGAGACTGAGCTGA (~60 bp);GENE SYNTHESIS STRATEGY;A yeast alanine transfer RNA (77 bp) (a) The chemical synthesis of 15 polydeoxynueleotide segments ranging in length from 5 to 20 nucleotide units (b) Ligase-catalyzed covalent joining of several segments to form three parts of the duplex, (c) Joining of the three parts to construct the entire duplex. ;ASSEMBLY PCR; The methods to synthesis the DNA with size around 2 kb are mature ;What is the next ?;Now we have 14 “perfect” 750-mers We need to assemble the 750-mers into one 10 kb piece We need them to go together in exactly the right order and orientation We need an “Assembly reaction”;On a limo to Milano, Solo gigolos don’t nod. Don’t nod, solo gigolos, On a limo to Milano;A typical “palindromic overhang” is generated by most restriction enzymes;View of the assembly reaction;Chemical synthesis the poliovirus, 2002;Flowchar