母种分离技术（The parent separation technique）.docVIP

母种分离技术（The parent separation technique） In nature, the bacteria do not exist alone, but live with many bacteria, radibacteria, and mold. The so-called germ separation is the separation of the bacteria that live together with the bacteria, and by cultivating them, they get pure good bacteria. Species in the denominator, species and cultivars. The program can refer to figure 13. (1) separation and culture of mother species The separation of edible fungi can be divided into spore separation method, tissue separation method and the separation of the substrate. 1. Spore separation The spore separation method is a method for the development of bacterial species by the germination of sexual spores or asexual spores of edible fungi. This species has a strong life force, but there is a difference between the individual spores, and the natural differentiation is more serious and the variation is large. It is necessary for the mushroom test to be applied in the production. (l) single spore separation method: it is a method for the mycelial species to be germinated into mycelium by taking only one of the bearing spores at each or every tube. The mushroom and the mushroom are separated by single spores and have strong ability, which can be used to separate and produce pure fungi. The single spore separation production is less used, and the technology is complex, and the polyspore separation method is generally used. (2) multi-spore separation method: it is a method to inoculate many spores on the same culture medium for germination and free mating to obtain the pure bacterial species of edible fungi. Specific operation methods include the following: Ejection method: (1) ZhongGu spores select individual robust, round shape is, no plant diseases and insect pests, the mushroom evenly, high and stable yield, strong adaptability of eight or nine points mature ZhongGu, cut off most, stalk with sterile water flushing, after several with sterilized gauze or surface moisture absorbent co