用Wathin-Starry银染色方法显示PM2 - 国家科技图书文献中心.DOC

用Wathin-Starry银染色方法显示PM2.5的实验证明 杨煦 岳常丽 王秦 黄宁华 徐东群 刘红刚 【摘要】目的 证实Warthin－Starry（WS）银特殊染色方法可较好地显示空气细颗粒物（fine particulate matter，PM2.5）。方法 将收集到的北京市游离的PM2.5制成混悬液，以不同剂量、采用支气管肺内滴入方式染毒大鼠，在不同时间取大鼠肺脏，除进行肉眼检查外，进行如下观察：⑴HE染色，⑵WS染色，⑶透射电镜（TEM）观察，⑷对肺内尘粒的X-线质谱元素分析（X-RSA），同时对游离的PM2.5标本也进行上述观察。对大鼠肺内肉芽肿的数目进行计数，对肺内尘粒的沉积程度测定其平均积分光密度值（IOD）和平均积分面密度值（IAD）。结果 随着染毒时间的延长和染毒剂量的增大，大鼠肺内肉芽肿的数目及其肉芽肿细胞质内尘粒的IAD值和IOD值也逐渐增高。在HE染色切片上，这些尘粒呈褐色，而在WS染色切片上尘粒呈黑褐色，更清晰，且出现了在HE染色切片上不被显示的微细颗粒，其IOD值和IAD值均显著高于HE染色下的测定值（P＜0.05），游离PM2.5的染色效果与此类似；TEM下大鼠肺内尘粒的超微结构与游离的PM2.5相同，X-RSA分析的结果两者的主要元素成分也相同，均为硅，进一步证实了大鼠肺内的尘粒即为被吞噬细胞吞噬的空气中游离的PM2.5。结论 WS银染色方法可以较好地显示人体细胞内的PM2.5，其染色效果优于HE染色。 【关键词】PM2.5；尘细胞；染色与标记 Experimental demonstration of Warthin-Starry silver staining method could show PM2.5 YANG Xu,, YUE Chang-li, WANG Qin, HUANG Ning-hua , XU Dong-qun, .LIU Hong-gang*, Department of Pathology, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, China.( YANG Xu, , YUE Chang-li, LIU Hong-gang,), Institute for Environmental Health and Related Products Safety, Chinese Center for Disease Control and Prevention, Beijing 100021, China. (WANG Qin, HUANG Ning-hua, XU Dong-qun,) *Corresponding author: LIU Hong-gang (liuhg1125@163.com), XU Dong-qun (dongqunxu@) 【Abstract】 Objective To prove Warthin-Starry(WS) silver staining method can show fine particulat matter(PM2.5). Methods The dissociative PM2.5 of Beijing city was collected to make. The rats were divided into different groups and exposed to different dosage of PM2.5 by intratracheal instillation. These rats were sacrificed at different times, sampled their lungs. After macroscopically observed, the pathological varieties and the situation of the dust particles in these rats’ lungs were observed using hematoxylin and eosin(HE) staining, Warthin-Starry(WS) silver stains, as well as the transmission of electron microscope(TEM), and x-ray spectrum chemical element analysis(X-RSA). Meanwhile the dissociative PM2.5 was observed by these same methods. We take count