防风多糖化学成分的研究.pdfVIP

第19卷第2期 化 学 研 究 Vo1．19 No．2 2008年6月 CHEMICAL RESEARCH Jun．2008 防风多糖化学成分的研究 王松柏，秦雪梅 ，刘焕蓉，郭小青，张丽增 (山西大学化学化工学院，山西太原030006) 摘 要：从中药防风Saposhnikovia divaricata(Turcz．)Schichk中分离得到两种酸性杂多糖SPSa和SPSb，经凝胶渗 透色谱证实为均一组分．uV_Vis光谱证实两种多糖中不含蛋白质．采用薄层色谱和毛细管电泳色谱确定了sP— sa和SPSb中单糖的组成及摩尔比，SPSa：半乳糖：阿拉伯糖：鼠李糖：半乳糖醛酸=1：2．3：0．15：4．8；SPSb：半乳 糖：阿拉伯糖：鼠李糖：木糖：半乳糖醛酸=1：1．5：0．8：0．2：10．2．采用IR光谱对多糖进行了初步结构研究．与 现有的防风多糖的组成相比较，确定出SPSa和SPSb为新的防风多糖． 关键词：防风；多糖；凝胶渗透色谱；薄层色谱；毛细管电泳色谱 中图分类号：O 629．12；Q 539；R 284．2 文献标识码：A 文章编号：1008—1011(2008)02—0066—03 Research for the Chemical Constituent of Saposhnikovia Divaricata(Turcz． Schichk Polysaccharides WANG Song—bai，QIN Xue—mei ，LIU Huan—rong，GUO Xiao—qing，ZHANG Li—zeng (School of Chemistry and Chemical Engineering，Shanxi University，Taiyuan 030006，Shanxi，China) AbstractTwo main acidic polysaccharides，SPSa and SPSb were separated from the the dried root and rhizome of Saposhnikovia divaricata(Turcz．)Schichk by the DEAE．Sepharose fast flow column chromat． agraphy．It was validated by gel permeation chromatography that they are homogenous and no protein was found from UV-Vis spectra．Component analysis and its molar ratio were ca~ied out by TLC and HPCE．The results show that SPSa mainly consists of galactose，arabinose，rhamnose and galacturonic acid in the molar ratio of l：2．3：0．15：4．8 and the SPSb mainly consists of galactose．arabinose。 rhamnose．xylose and galacturonic acid in the molar ratio of 1：1．5：0．8：0．2：10．2，respectively．Pri— mary structure was studied bv IR．It can be drawn a conclusion that SPSa and SPSb are novel acidic po． 1ysaccarides from Saposhnikovia divaricata(Turcz．)Schichk． Keywor