交替氧化酶通过控制ROS的产生来影响植物对生物胁迫的反应.pptVIP

Alternative oxidase impacts the plant response to biotic stress by influencing the mitochondrial generation of reactive oxygen species PLANT CELLENVIRONMENT 4.666 introduction inoculation of tobacco with Pseudomonas syringae incompatible pv. maculicola results in a rapid and persistent burst of superoxide (O2-) from mitochondria, no change in amount of mitochondrial alternative oxidase (AOX) and induction of the hypersensitive response (HR). However, inoculation with incompatible pv.phaseolicola resulted in increased AOX, no O2-burst and no HR. Plants were grown in controlled environment chambers (Model PGR-15, Conviron, Winnipeg, MB, Canada) with a 16 h photoperiod, temperature of 28 °C/22 °C (light/dark), relative humidity of 60%, photosynthetic photon flux density of 130 mmol m-2s-1, Plants were irrigated with water daily and fertilized with 10x diluted Hoagland’s solution three times per week. Plants were used 5–6 weeks after initiating germination in vermiculite. density was adjusted to 1 *107cfu mL-1 for plant inoculations. Plants were inoculated at 5 h into the light period. Mock-inoculated plants were infiltrated with distilled water. the absolute level of H2O2 was not statistically different between the WT and transgenic lines after inoculation, owing it seems to the differences in the initial level of this ROS species between WT and transgenic plants. discussion the level of AOX is an important determinant of the mitochondrial O2- burst O2- burst is not, in itself, sufficient to generate the HR. This is not surprising and is consistent with hypotheses that the HR is dependent upon multiple coordinating signals that are invoked in response to some incompatible interactions the mitochondrial O2-burst does promote the HR The O2-burst normally seen towards pv. maculicola is delayed in plants lacking AOX. This delay is associated with a delayed HR, suggesting that the burst does promote the HR. the mitochondrion is a target of biotic stress A O2-burst