桔梗皂苷d对氧化型低密度脂蛋白诱导的内皮细胞氧化损伤 - 食品科学.pdf

※营养卫生 食品科学 2013, Vol.34, No.13 293 桔梗皂苷D对氧化型低密度脂蛋白诱导的 内皮细胞氧化损伤的作用 王茂山，吴敬涛 (济南大学食品科学与营养系，山东 济南 25000 1) 摘 要：为检测桔梗皂苷D对氧化型低密度脂蛋白(OxLDL)诱导的内皮细胞氧化损伤的作用，利用OxLDL诱导人脐 静脉内皮细胞(HUVECs) ，建立细胞氧化损伤模型，分别以0.5 、0.75 、1mg/mL桔梗皂苷D(PD)处理，测定培养液一 氧化氮(NO)和丙二醛(MDA)水平；实时荧光定量聚合酶链式反应(qRT-PCR)检测细胞动脉细胞黏附分子(VCAM- 1) 和细胞黏附分子(ICAM- 1) 的表达；同时进行单核细胞与内皮细胞的黏附实验。结果表明：PD能显著增大细胞培养 液NO浓度(P ＜0.0 1) ，显著降低MDA水平(P ＜0.0 1) ，减少VCAM- 1和ICAM- 1分子的表达(P ＜0.0 1)及单核细胞与内 皮细胞的黏附作用(P ＜0.0 1) 。说明PD能抑制OxLDL诱导的内皮细胞氧化损伤。 关键词：桔梗；桔梗皂苷D ；氧化损伤；细胞黏附 Effect of Platycodin D on OxLDL-induced Oxidative Injury of Endothelial Cells WANG Mao-shan ，WU Jing-tao (Department of Food Science and Nutrition, University of Jinan, Jinan 25000 1, China) Abstract ：The effect of platycodin D (PD) on human umbilical vein endothelial cells (HUVECs) were evaluated in vitro. OxLDL at 50 mg/L was used to induce oxidative injury in HUVECs to establish a cell model of oxidative injury. The cells were pre-treated with various concentrations of PD (0.5, 0.75 mg/mL and 1 mg/mL). The levels of nitric oxide (NO) and malonaldehyde (MAD) in the culture medium were determined. The mRNA expression of vascular cell adhesion molecule-1 (VCAM-1) and intercellular cell adhesion molecule-1 (ICAM-1) in endothelium cells were also analyzed as well as the adhesion of monocytes to endothelial cells. The results showed that PD treatment significantly increased NO levels (P ＜0.01) but decreased (P ＜0.01) MDA levels in the medium of endothelial cells. Moreover, PD significantly downregulated mRNA expression levels of VCAM-1 and ICAM-1 in endothelial cells (P ＜0.01) but also inhibited the monocyte adhesion to endothelial cells (P ＜0.