水芹的组织培养及快速繁殖研究.pdfVIP

安徽农业科学，Journal ofAnhui A ．Sci．2008，36(14)：5761—5762，5800 责任编辑 张杨林 责任校对 卢瑶 水芹的组织培养及快速繁殖研究 许文一，高洋，马依妮，孙慧超，姜长阳 (辽宁师范大学生命科学学5完，辽宁大连l16021) 摘要 [目的]更好保护野生植物资源水芹，并为其人工栽培提供依据。[方法]以水芹嫩茎为外植体，研究水芹愈伤组织的诱导和分 化及试管苗生根、移栽、扦插的条件。[结果]MS+BA 0．5 mg／L+NAA l mg／L是诱导水芹嫩茎形成具有分化能力愈伤组织的最佳培养 基。MS+AgN0 0．4mg／L+BA 0．2mg／L是诱导水芹愈伤组织和不定芽分化的最佳培养基。1／3MS+IAA 0．6mg／L是水芹不定芽生根培养 和生根继代培养的最佳培养基。以炉灰痊为试管苗的移栽扦插基质，移栽成活率为99％，扦插成活率为94％。移栽和扦插的水芹试管 苗具有生长快和长势旺的特点，90 d收获的鲜食茎叶比野生水芹增产15％，根系为野生植株的2倍。[结论]在生产上，应采用试管苗 生根继代培养的方法进行水芹的快速繁殖。 关键词 水芹；愈伤组织；无性系；快速繁 中图分类号 $336 文献标识码 A 文章编号 0517—661l(2008)14—05761—02 Study on the Tissue Culture and Rapid Propagation of Oenanthejavanica(Blume)DC． XU Wen_vi et al fCollege of Life Science，Lia0ning Normal University，Dalian，Liaoning 1 16021) Abstract 『Objective]The research aimed to protect wild plant resource Oenanthe avanica(Blume)DC．better and provide basis for its artificial planting．『Method]With the tender stem of 0． cwolT,ica as explant，the conditions of the callus induction and differentiation，the rooh’ng， transplanting and cutting of test-tube seedling in O． ∞ c0 were studied．[Result]The optimum medium for inducing the tender stem of 0． cwolT,ica to f0rm the tissue with the differentiation ability was MS+BA 0．5 m扎 +NAA lmg／L．The optimum medium for inducing the differentiation of the callus and adventitious buds in 0． cwotl,ica was MS+AgNO 0．4 mg／L+BA 0_2 mg／L．The optimum medium for the rooting culture of the adventitious buds and the rooting subculture in 0． avanica was 1／3 MS+IAA 0．6 mg／L．When the stove ash and slag were taken as the transplanting and cutting matrix of test-tube seedling．the transplanting survival rate was 99％ and the cutting survival rate was 94％．The transplanted and cut test-tube seedling of O． avanica had the characteristics of fast growth and good growth vigour．The fresh-eating stems and leaves harvested on the 90th d were increased 15％than wild O． anica and the root was twic