胚胎晚期母体暴露细菌脂多糖对中年CD-1子鼠背.PDFVIP

安徽农业大学学报, 2012, 39(2): 174-176 Journal of Anhui Agricultural University 网络出版时间：2012-02-29 14:18 [URL] /kcms/detail/34.1162.S1418.004.html 胚胎晚期母体暴露细菌脂多糖对中年 CD-1 子鼠背 海马 synaptotagmin Ⅳ的影响 1 1 2 1 1 1* 卞文婧 ，杨启纲 ，王龙海 ，王 芳 ，童晶晶 ，陈贵海 (1. 安徽医科大学第一附属医院神经内科，合肥 230022 ；2. 安徽中医学院解剖学教研室，合肥 230038) 摘 要：对胚胎晚期母体腹腔注射细菌脂多糖或生理盐水，利用免疫组化法对CD-1 中年子鼠背海马CA1 、CA3 区和齿状回各层突触蛋白synaptotagmin IV 的表达进行研究。结果显示synaptotagmin IV 在背海马各层均有表达。 与盐水对照组相比，细菌脂多糖处理组子鼠背海马CA1 、CA3 区各层和齿状回分子层的synaptotagmin IV 含量显著 增高（Ps 0.05 ）。结果表明胚胎晚期母体暴露细菌脂多糖可显著增加中年子鼠背海马Syt IV 表达。 关键词：衰老；细菌脂多糖；小鼠；海马；synaptotagmin IV 中图分类号：S865.13 文献标识码：A 文章编号：1672−352X (2012)02−0174−03 Effects of maternal exposure to lipopolysaccharide during late pregnancy on the level of synaptotagmin IV in dorsal hippocampus of middle-aged offspring CD-1 mice 1 1 2 1 1 1 BIAN Wen-jing , YANG Qi-gang , WANG Long-hai , WANG Fang , TONG Jing-jing , CHEN Gui-hai (1. Department of Neurology, the First Affiliated Hospital of Anhui Medical University, Hefei 230022; 2. Department of Anatomy, Anhui College of Traditional Chinese Medicine, Hefei 230038) Abstract: In the present study, by means of immunohistochemical method, the contents of synaptotagmin IV in the CA1, CA3 and dentate gyrus subfields of dorsal hippocampus were measured in the middle-aged CD-1 mice, whose mothers were intraperitoneally injected with lipopolysaccharide or normal saline during late preg- nancy. The results indicated that the synaptotagmin IV was expressed in each stratun of all subfields in the dorsal hippocampus. Compared to the saline-treated group, the