超声辅助乳化液相微萃取分析绿茶饮料和番茄汁中有机磷农药.PDFVIP

DOI: 10.7524/AJE.1673-5897.20170112004 赵越, 熊亚兵, 杨中华, 等. 超声辅助乳化液相微萃取分析绿茶饮料和番茄汁中有机磷农药 残留[J]. 生态毒理学报, 2017, 12(3): Zhao Y, Xiong Y B, Yang Z H, et al. Analysis of organophosphorus pesticide residues in green tea and tomato juice by ultrasound-assisted emulsification microextraction(USAEME) [J]. Asian Journal of Ecotoxicology, 2017, 12(3): (in Chinese) 超声辅助乳化液相微萃取分析绿茶饮料和番茄汁中有机磷农药残留 * 赵越，熊亚兵，杨中华 ，李建洪 华中农业大学，武汉，430070 收稿日期：2017-01-12 录用日期：2017-03-13 摘要：农药施用于田间，必然会残留于环境中。农药残留的分析方法多种多样。为探究灵敏 度高、重现性好的样品前处理方法，建立了超声辅助乳化液相微萃取，结合气相色谱和火焰 光度检测器测定绿茶饮料和番茄汁中5 种有机磷农药的方法，对萃取条件进行了优化，如萃 取溶剂种类和体积，超声频率和时间，盐效应。在优化后的萃取条件下，5 种有机磷农药实 现良好分离，空白添加实验结果表明在0.1~10 μg·L-1 ，线性相关系数在0.9985~0.9994 之间， LOD 在0.005~0.020 μg·L-1 之间，RSD(n=5)在4.3%~9.4%之间。在绿茶饮料和番茄汁实际样 本检测中，目标分析物含量均低于检出限。样本添加回收试验的回收率均大于 88.5% ， RSD(n=3)在2.2%~9.8% ，结果表明该方法用于液体样本中农药残留检测真实可靠。 关键词：有机磷农药; 绿茶饮料; 番茄汁; 超声辅助乳化液相微萃取 文章编号： 中图分类号： 文献标识码：A Analysis of Organophosphorus Pesticide Residues in Green Tea and Tomato Juice by Ultrasound-Assisted Emulsification Microextraction (USAEME) * Zhao Yue, Xiong Yabing, Yang Zhonghua , Li Jianhong Huazhong Agricultural University, Wuhan 430070, China Received 12 January 2017 accepted 13 March 2017 Abstract: The pesticide residue will inevitably exist in the environment after the application of pesticides in the field. There are various pesticide residue analysis methods applied in different pesticides and conditions tests. In order to explore the method of sample pretreatment with high sensitivity and good reproducibility, Ultrasound-assisted Emulsification Microextraction (USAEME) were developed. The method of gas chromatography and the flame photometric detector was used to determine five kinds of organophosphorus pesticides in green tea beverage and to