High-Performance Liquid Chromatography（高效液相色谱法）.pdfVIP

High-Performance Liquid Chromatography High Performance Liquid Chromatography (HPLC) was developed in the late 1960s and early 1970s. Today it is widely applied for separations and purifications in a variety of areas including pharmaceuticals, biotechnology, environmental, polymer and food industries. HPLC has over the past decade become the method of choice for the analysis of a wide variety of compounds. Its main advantage over GC is that the analytes do not have to be volatile, so macromolecules are suitable for HPLC analysis. HPLC is accomplished by in