关于MapmakerExp遗传作图中标记分群和排序操作技术的讨论.pdfVIP

作物学报 ACTA AGRONOMICA SINICA 2008, 34(2): 217−223 /zwxb/ ISSN 0496-3490; CODEN TSHPA9 E-mail: xbzw@ DOI: 10.3724/SP.J.1006.2008.00217 关于Mapmaker/Exp 遗传作图中标记分群和排序操作技术的讨论 邢光南 赵团结 盖钧镒* (//, 210095) : Mapmaker/Exp (3.0), ( 500 ) (1)LOD , LOD ; (2), order ripple , order, order ripple , , 2 QTL , : Mapmaker/Exp (3.0); ; ; ; Application Technique of Marker Grouping and Ordering in Genetic Linkage Map Construction Using Mapmaker/Exp * XING Guang-Nan, ZHAO Tuan-Jie, and GAI Jun-Yi (Soybean Research Institute of Nanjing Agricultural University, National Center for Soybean Improvement, National Key Laboratory for Crop Ge- netics and Germplasm Enhancement, Nanjing 210095, Jiangsu, China) Abstract: Mapmaker/Exp (3.0) is one of the most often used computer software packages for constructing genetic linkage map worldwide. It was found that the distances of the map constructed with the software were often exaggerated when large number of markers was involved. The present paper is to introduce our technical experiences in using Method 2 (marker number more than 500) of the software for a more reasonable linkage map. In Method 2 of Mapmaker/Exp (3.0), markers were usually grouped into linkage groups under an identical LOD value for all linkage groups; and then an “order” command followed by multiple “ripple” command