依托咪酯对大鼠海马CA1区全细胞自发性兴奋性突触后电流的影响 于 .DOC

依托咪酯对大鼠海马CA1区全细胞自发性兴奋性突触后电流的影响 于婵娟 刘亚华 侯彦深 史春慧 蒋晖 新疆医科大学附属肿瘤医院麻醉科 830011 [摘要]目的：观察依托咪酯对大鼠海马CA1区自发性兴奋性突触后电流（sEPSC）的影响。方法将wistar大鼠海马脑片分为脂肪乳剂组、10μmol/L依托咪酯组。10min sEPSC为基础值，加入脂肪乳剂或依托咪酯后30～35minsEPSC作为指标，观察两组sEPSC频率、幅值、半衰期以及上升时间的变化。膜钳制电压为-70mV。结果：在膜钳制电压为-70mV条件下，10μmol/L依托咪酯不改变sEPSC的频率，而10μmol/L依托咪酯sEPSC的幅值，半衰期明显降低。sEPSC的上升时间明显缩短。结论：静息状态下，依托咪酯脂肪乳剂通过突触后膜抑制自发性兴奋性突触活动。 [关键词]： 自发性兴奋性突触后电流 依托咪酯 海马 Effects of etomidate on spontanous excitatory postsynaptic current in hippocampal CA1 region of rats YUN Chanjun, LIU Yahua, Hou Yanshen,Shi Chunhui,Jiang Hui et al. Department of Anesthesiology, Affiliated Tumor Hospital, Xinjiang Medical University, Urumqi 830011, CHINA Abstract:Object: Observe the effect of etomidate emulsion on spontanous excitatory postsynaptic current (sEPSC) of hippocampal CA1 region of rats. Methods: The 20 hippocampal slices were evenly divided into two groups. They were emulsion group, etomidate of 10μmol group. By whole-cell voltage-clamp technique, Record the 10 min sEPSC as the base value, the effects of 10μmol/L etomidate emulsion on sEPSC were observed. Results: At a holding potential of-70mV,10μmol/L etomidate emulsion didn’t change the frequency of sEPSC in the hippocampal CA1 neurons of the rat, but markedly reduced the amplitude of sEPSC from 16.012 pA to 11.168 pA. There were obvious differences between the two groups in amplitude, halflife (decreased from 15.206 ms to 8.635 ms), rise time (shortened from 5.402 ms to 4.325ms)(P0.05).Conclusion: In quiet condition, etomidate emulsion inhibits spontaneous excitatory synaptic transmission through the postsynaptic membrane in the CA1 pyramidal neurons of hippocampus. Key Word: spontanous excitatory postsynaptic current ； Etomidate；Hippocampus； [1,2]。仅管临床研究报道很多，对于依托咪酯发挥麻醉作用的细胞和分子机制尚不清楚。 海马锥体细胞的突触生理表明，静息状态下，突触前膜释放的谷氨酸呈量子式释放到突触间隙，然后与突触后膜的相应受体如α-氨基-3-羟基-5-甲基恶唑-4-丙酸(AMPA)及N-甲基-D-门冬氨酸(NMDA)等受体结合引起突触后膜产生去极化电流，即自发性兴奋性突触后电流(spontanous excitatory postsynaptic current，sEPSC)[3,4]。也就是说，sEPSC反映兴奋性突触在不受外界刺激