HPLC法测定何首乌中二苯乙烯苷含量.docVIP

HPLC法测定何首乌中二苯乙烯苷含量[摘要] 目的:建立高效液相色谱法(HPLC)测定何首乌中二苯乙烯苷含量的方法。方法:采用高效液相色谱法(HPLC),以十八烷基硅烷键合相硅胶为固定相(4.6 mm ×250 mm,5 μm),色谱纯乙腈-水(25∶75),检测波长325 nm,流速为1.0 ml/min,色谱柱柱温30 ℃,测定何首乌中主要成分二苯乙烯苷的含量。结果:何首乌中二苯乙烯苷进样量在0.200～4.000 μg 范围内,与峰面积呈良好的线性关系,其回归方程为Y=152.92X+45.67(r=0.9997);平均回收率为99.96%。结论:该方法操作简单、方便、准确、重复性好,用于测量二苯乙烯苷的含量可信度高。 [关键词] 何首乌;二苯乙烯苷;高效液相色谱法;含量测定 [中图分类号] R283 [文献标识码]B [文章编号]1674-4721(2010)06(c)-041-02 HPLC Determination of stilbene glucoside in polygonum multiflorum content determination of content of 2, 3, 5, 4’-tetrahydroxystilbene-O-β-D-glucoside in radix polygoni multiflori SU Lijie (Chinese Medicine Hospital of Xiangtan City, Hunan Province, Xiangtan 411100, China) [Abstract] Objective: To establish a method for the determination of 2, 3, 5, 4’-tetrahydroxystilbene-O-β-D-glucoside in radix polygoni multifloriby HPLC. Methods: The condition of HPLC was KromasiL C18 column (octadecylsilan reversed bonded phase, 4.6 mm×250 mm, 5 μm), mobile phase of chromatographic purity acetonitrile:water (25:75) and UV dector at 325 nm. The flow rate was 1.0 ml/min and the column temperature was 30℃. Results: The method proved to be linear in the range at 0.200-4.000 μg with correlation coefficient of 0.999 7. The linear formula was Y=152.92X+45.67. The average recovery and recision were 99.96%. Conclusion: The method is simpLe, accurate, and can be a effective method to control the quality. [Key words] Radix polygoni multiflori ; 2, 3, 5, 4’-tetrahydroxystilbene-O-β-D-glucoside; HPLC; Assessment 何首乌为蓼科植物何首乌(polygonum multiflorum thunb)的干燥块根,具有补肝肾、益精血、乌须发、壮筋骨、解毒、消痛、润肠通便之功效[1]。何首乌中二苯乙烯苷类成分在抗衰老、抗老年痴呆、增强机体免疫、造血和改善心血管功能等方面有许多独到之处[2]。本文采用高效液相色谱法,对二苯乙烯苷类的含量测定进行了方法学方面的考察试验。该方法操作简单、方便、重复性好,用于测定含量可信度高,效果满意。 1 仪器与试剂 1.1 仪器 Waters高效液相色谱仪;Empower 色谱工作站;KromasiL C18 反相柱( 4.6 mm ×250 mm,5 μm);纯水器。 1.2 试剂 纯水,溶剂均为分析纯;流动相为色谱纯,2,3,5,4-四羟基二苯乙烯-2-O-β-D-葡萄糖苷对照品,简称二苯乙烯苷,购于中国药品生物制品检定所。 1.3 试验材料 何首乌药材干粉碎过60目筛,于60°C干燥至恒重后置干燥器中备用。 1.4 高效液相色谱条件 色谱柱:Kroma