原花青素对大鼠脑缺血再灌注损伤抗炎机制探究.docVIP

原花青素对大鼠脑缺血再灌注损伤抗炎机制探究【摘要】 目的 研究原花青素（GSP）对大鼠局灶性脑缺血再灌注损伤抗炎作用机制。方法 大鼠脑缺血再灌注模型完成后，缺血2 h再灌注24 h，其中原花青素大剂量组(40 mg/kg)、小剂量组(10 mg/kg)术前7 d分别腹腔注射2 ml/kg GSP，模型组腹腔注射2 ml/kg生理盐水，1次/d，再灌注前15 min加注1次。观察不同剂量的原花青素对脑组织一氧化氮（NO）含量、髓过氧化物酶（MPO）活性和脑组织神经细胞的细胞间黏附分子-1（ICAM-1）蛋白的表达的影响。结果 原花青素可显著减轻大鼠脑缺血再灌注损伤的行为评分；显著降低脑组织中NO含量和MPO的活性，差异有统计学意义（P0．05）；ICAM-1蛋白的阳性表达率降低，差异有统计学意义（P0．05）。结论 原花青素对大鼠脑缺血再灌注损伤有保护作用，作用机制之一可能与其抑制炎性反应有关。? 【关键词】原花青素；脑缺血再灌注损伤；细胞间粘附分子-1；髓过氧化物酶；一氧化氮 The study of anti-inflammatory mechanisms of procyanidin on cerebral ischemia reperfusion injury of rats WANG Feng,GUO Jian ? 【Abstract】 Objective To investigate the Anti-Inflammatory mechanisms of grape seed procyanidin (GSP) on cerebral ischemia reperfusion injury (CIRI) of rats.Methods The model of MCAO was established by intraluminal suture technique.After 2 h of occlusion and 24 h of reperfusion，The high dose GSP (40 mg/kg) group and low dose GSP (10 mg/kg) group.was injected by intraperitoneal injection for 7 days ，and all groups were given a more time in 15 minutes before operation.The contents of NO and activities of MPO in brain tissue of ischemic hemisphere were measured; the expression of ICAM-1 in the cortex were measured by immunohistochemical method.Results The neurological scores of rats treated with GSP was reduced apparently compared with model group rats (P0．05); there were apparent reduction of the contents of NO and marked decrease of MPO in GSP group compared with the I/R model group(P0．05); compared with I/R model group，the expression of ICAM-1 was down-regulated obviously in GSP group (P0．05).Conclusion GSP could relieve the cerebral ischemia reperfusion injury，one of the mechanisms could be the Anti-Inflammatory effect of procyanidin ? 【Key words】Procyanidin;CIBI;ICAM-1;NO;MPO ? 原花青素是一类从葡萄籽中提取出的天然植物多酚化合物，具有极强的抗氧化、清除自由基、抗炎、减轻缺血再灌注损伤[1]降低血小板凝聚[2]等保护作用。本试验研究目的旨在探讨GSP在体内对脑缺血再灌注损（CIRI）伤的保护作用及抗炎机制，从而为临床脑保护药物的研制和开发提供实验依据。? 1 材料与方法? 1．1 材料 健康Wistar大鼠64只，体质量250~300 g，雄性，由山东鲁抗集团动物中心提供。原