溶解细胞.PDF

警告： 本课程所介绍的实验具潜在地危险并需完整地安全训练、特别的设备及仪器，并需有适当的人员于 一旁监督。你必须为实施这些安全措施及方法承担唯一的责任、义务及危险性。麻省理工学院无须 为所提供的教材或实施所提供的数据担负任何责任、义务及危险性。 GUS 分析 摘自The Quasi Definitive Guide to Determining β–Glucosidase Activity in Cell Lysates (Special bonus protocols for the harvesting and lysis of E.coli, Rhodococcus and Corynebacterium) by Jennie Cho, (modified and spiffed up by xian o’brien, selflessly edited by Phil Lessard) 摘自Wilson et al., The Escherichia coli gus Operon: Induction and Expression of the gus Operon in E. coli and the Occurrence and Use of GUS in Other Bacteria. From GUS Protocols: Using t