肺结核患者外周血CD4+CD25+调节性T细胞检测及意义.docVIP

肺结核患者外周血CD4+CD25+调节性T细胞检测及意义摘要:[目的] 通过对肺结核患者外周血中CD4+CD25+调节性T细胞的分析,探讨其在肺结核患者中的临床意义。 [方法] 采用流式细胞技术多色荧光分析法,测定84例肺结核患者及29例健康体检者外周血中CD4+CD25+调节性T细胞水平,观察肺结核患者与健康对照者之间、各型肺结核之间及结核杆菌阳性与阴性者之间的差别。 [结果] 肺结核患者CD4+CD25+所占比例为(10.81±2.65)%,与健康对照组差异有统计学意义(P0.05?)。 [结论] CD4+CD25+调节性T细胞可作为判断肺结核患者免疫功能的指标,它对肺结核患者的免疫状态、指导用药、疗效观察有一定的临床意义。 关键词: 肺结核; 调节性T淋巴细胞; CD4+CD25+T细胞 中图分类号:R 521 文献标志码: A??? CD4+CD25+ regulatory T cell in peripheral blood of patients with pulmonary tuberculosis: Its detection and clinical significance LIU Hong-mei (The Sixth People?s Hospital of Shaoxing, Zhejiang 312000 China) Abstract:[Objective] To analyze CD4+CD25+ regulatory T cell (Treg) in the peripheralblood of patients with pulmonary tuberculosis and explore its clinical significance. [Methods] A series of 84 pulmonary tuberculosis patients were detected by flow cytometry with multicolor fluorescence technology for CD4+CD25+ regulator T cell, with 29 healthy persons as control group.The differences were analyzed betweentypes of pulmonary tuberculosis, bacteriologically positive and negative, initial treatment and retreatment. [Results] The percentage of CD4+CD25+ regulator T cell of pulmonary tuberculosis patients (10.81±2.65) wassignificantly higher than that of controls (P0.05). [Conclusion] CD4+CD25+ regulator T cell can be used as indicator of the immune function in pulmonary tuberculosis patients.It is of clinical significance for theobservationofimmune status,medication andcurative effect on pulmonary tuberculosis patients. Key words: Pulmonary tuberculosis; Regulatory T cell; CD4+CD25+T cell 结核是T淋巴细胞介导的细胞免疫。调节性T细胞参与自身免疫、肿瘤免疫和其他传染性病原体的免疫过程,这些细胞在结核杆菌引起的免疫抑制中发挥作用。我们采用流式细胞仪检测了肺结核患者外周血CD4+CD25+调节性T细胞(Treg细胞),探讨肺结核患者CD4+CD25+Treg细胞介导的细胞免疫与疾病发展的关系,为肺结核的免疫治疗提供相关依据。?? 1 材料与方法? 1.1 对象 肺结核组为我院2007年11月至2008年6月住院的84例肺结核患者,均根据临床症状、体征、X线胸片、CT扫描、痰菌检查确诊,其中男性51例,女性33例,年龄28～78岁,Ⅲ型结核66例,Ⅳ型结核18例;对照组均为健康体检者,共29例,其中男性18例,女性11例,年龄20～67岁。? 1.2 试剂和仪器 所用仪器为Beckman-Coulter Epics