芹菜对雌性小鼠动情周期影响.docVIP

芹菜对雌性小鼠动情周期影响【摘要】 目的 探讨芹菜对雌性小鼠动情周期的影响。方法 对未处理的100只小鼠采用阴道脱落细胞涂片法观察动情周期。将动情周期规律的小鼠随机分为：对照组、低剂量组、中剂量组和高剂量组，对照组灌生理盐水、剂量组分别灌不同剂量的芹菜汁，每日观察动情周期。小鼠于灌胃7 d、14 d分批处死。结果 灌胃14 d高剂量组小鼠动情前期较灌胃前明显缩短（P＝0．002），动情后期则较灌胃前延长（P=0．018）。结论 该剂量的芹菜汁可导致雌性小鼠的动情周期的紊乱。? 【关键词】 芹菜；动情周期；血红蛋白；雌鼠?? The Effects of Celery on the Estrous Cycle of Female Mice AN Jing, MA Rui-Lan, PU Yu-dong,et al.School of Public Health of Lanzhou University, Lanzhou, 730000,China? 【Abstract】 Objective To study the effects of Celery on Estrous Cycle of Female Mice.Methods We observed one hundred female mice estrous cycles by vagina exfoliative cytoscopy examination every morning and chose female mice with regular estrous cycle. They were randomly divided into four groups, i.e., control group,low dose group,medial dose group and high dose group. Experimental group were been exposed to celery by oral administer every day, and the doses was 0.3 ml. The control group were been exposed to normal saline by oral administer and the doses was 0.3 ml. Female mice vagina exfoliative cytoscopy was observed at some time. Results After being exposed to celery by high dose for 14 days, the proestrus was shortened (P＝0．002), and the metestrus was prolonged (P=0．018).Conclusion The result suggested that celery had been making disturbance of estrous cycles.? 【Key words】 Estrous Cycle; Celery; Haemoglobin; Female Mice 芹菜是一种常见的蔬菜，它不仅可以做菜，又可供药用，故又有“药芹”之称。芹菜中富含三羟基黄酮类化合物（芹菜素），其作为一种植物性雌激素单独使用时表现出雌激素的性质，与乙炔基雌二醇联用则表现较弱的抗雌激素的性质[1]。? 虽然芹菜早已引起了人们的关注[2]，但是芹菜对于生殖系统影响的报道很少。本研究采用芹菜汁灌胃的方法观察芹菜对于雌鼠动情周期的影响，旨在探讨芹菜对小鼠生理机制的影响，为芹菜开发利用提供一定的依据。? 1 材料与方法? 1．1 实验动物 SPF级健康成年昆明种雌性小鼠100只，体重29～33 g，周龄8周，（实验动物由甘肃省中医学院动物实验中心提供）。动物合格证号为：scxk(甘)004-007。? 1．2 芹菜汁的制备 称取市售的西芹，摘掉黄叶，洗净、晾干、切碎 、称重（1 125 g）后，采用多功能榨汁机分次粉碎榨汁，用四层消毒纱布过滤菜汁，除去杂质，共得芹菜汁590 ml；然后将原液160 ml封存、冷冻备用，即为低剂量组（浓度约为1．907 g/ml）；将其余芹菜汁以20 ml为单位分别放入直径9 cm的平皿中，然后将平皿置于50℃水浴箱中分别蒸发到原体积的1/2和1/4，待冷却后封存、冷冻备用，即得中剂量组（浓度约为3．814 g/ml）和高剂量组（浓度约为7．628 g/ml）。? 1．3 小鼠动情周期的观察 参照施新猷的《医学实验动物方法》，每天早上8点开始给小鼠