牵张负荷对大鼠膀胱Cajal间质细胞超微形态及c-kit基因表达影响 路扬.DOC

牵张负荷对大鼠膀胱Cajal间质细胞超微形态及c-kit基因表达影响 路扬,李龙坤,王永权(第三军医大学西南医院全军泌尿外科研究所,重庆400038) 摘要：目的 通过研究膀胱Cajal间质细胞(Interstitial cells of cajal，ICCs )超微形态及c-kit基因水平变化，阐明牵张负荷对膀胱Cajal细胞兴奋性的调控作用。方法　结扎膀胱颈，构建大鼠膀胱出口部分梗阻致逼尿肌不稳定模型，通过免疫电镜方法观察牵张负荷条件下逼尿肌不稳定组与正常对照组膀胱ICCs细胞超微形态变化，观察牵张负荷条件下逼尿肌稳定组和逼尿肌不稳定组与正常对照组膀胱ICCs细胞酪氨酸激酶受体(c-kit)在基因水平表达差异。结果 逼尿肌不稳定组ICCs细胞形态和c-kit基因水平表达量较正常对照组有明显改变(P0.01)。结论 牵张负荷对ICCs细胞有调控作用，可能的作用机制在于改变ICCs细胞形态及上调c-kit 表达。 关键词 牵张负荷 ICCs细胞 c-kit Effects of stretch load on the ultramicrostructure and c-kit genetic expression of the bladder ICC in rats Lu Yang，Li Long kun，Wang Yong quanUrinary Surgery Research Institute, Southwest Hospital, Third Military Medical University, Chongqing 400038, China) [Abstract] Objective To investigate the effects of stretch load on the ultramicrostructure and c-kit expression of the bladder ICCs in rats. Methods Rats with detrusor instability due to partial outflow obstruction with bladder neck ligation were established. The effects of stretch load on the changes of ultramicrostructure of the bladder ICCs with immunoelectron microscope. The variations of c-kit genetic expression of ICCs in stable, unstable and normal detrusor were analyzed with RT-PCR. Results The ultramicrostructure of the ICCs was significantly changed, and its expression of c-kit was significant higher in the unstable group than that in stable and normal groups (p0.01). Conclusions The stretch load can regulate the function of ICCs, with the possible mechanism of variate the ICCs morphiology and up-regulate its c-kit expression. [key words] stretch load; interstitial cells of Cajal; c-kit *逼尿肌兴奋性异常是临床上膀胱功能障碍性疾病的主要内在原因之一，了解逼尿肌兴奋性起源和调控对于膀胱兴奋性异常的基础研究和临床诊治有相当重要意义。研究表明：在离体条件下，膀胱逼尿肌失去神经支配时，受到牵张负荷后，逼尿肌细胞仍然可产生自发性兴奋，启动逼尿肌肌条收缩[1]。在前期的研究中，通过c-kit免疫组织化学染色方法，已经证实成年大鼠膀胱存在有与胃肠道相同的Cajal间质细胞(interstitial cells of Cajal,ICCs)，并且在给予一定的牵张负荷后，ICCs细胞数量及功能有明显改变[2]。说明ICCs细胞应该是兴奋起源和传导的中枢，而牵张负荷对逼尿肌的兴奋性有调控作用，本研究拟进一步探讨逼尿肌不稳定(detrusor instability,DI)时，ICCs细胞超微结构的变化及c-kit在基因水平表达差异，阐明ICCs细胞在膀胱兴奋性的作用，以及牵