微生物絮凝剂产生菌的筛选及絮凝活性研究+.PDFVIP

第26卷 第6期 重庆建筑大学学报 V01．26 Nc．6 2004年 l2月 Journal of Chongqing Jianzhu Universi Dec．2004 微生物絮凝剂产生菌的筛选及絮凝活性研究+ 罗 平 ， 罗固源2， 邹小兵 (1．重庆大学 化学化工学院，重庆 4OOO44；2．重庆大学 城市建设与环境工程学院，重庆 400045) 摘要：用富集一分离一筛选的方法从花园土壤中分离纯化得到两株具有较高絮凝活性的絮凝 剂产生菌。通过研究2B菌株在不同培养时间的生长情况、培养液中pH值变化情况及絮凝活 性等，从而得出絮凝活性与菌生长量成正相关，且该菌产生的絮凝剂对高岭土悬浊液具有较高 的絮凝活性和稳定性。环境因素对絮凝剂产生的影响研究表明，2B菌株产絮凝刺的最佳培养 基为查氏培养基；菌产絮凝剂的最佳培养条件：初始 pH值为7～9，培养温度 30 c【=，培养时间 为72 h，通气量为 l50 r／min。研究表明，2B菌株所产絮凝剂絮凝高岭土悬浊液的最适宜pH在 大于7的偏碱性条件下，ca2 、M 和 Al 等金属离子对其絮凝具有促进作用，絮凝率可高达 94．6％ 关键词：微生物絮凝剂；絮凝刺产生菌；絮凝活性；时间曲线 中图分类号：X506 文献标识码：A 文章编号：1006—7329(2004)06—0063—04 Screening of Flocculants——producing Strains and Study 011 Flocculating Activity LUO Ping ，LUO Gu—yuan2，ZOU Xiao—bing (1．Co~ege of Chemistry and Chemical Technology，Chongqing University，Chongqing 4OOO44，P．R．Claim；2．College of Urban Con— struetion and Environmental Englneering，Chongqing University，Chongqing 400045，P．R．Chi~) Abstract：Two flocculants—producing strains of hi gh flocculating activity were screened from the soil by the method of enrichment—isolation—screening．The growth of the strains．flocculating activity and pH at different time during the culture of the strains was studied and four different kinds of media were selected to culture the microorganisms． It is found that zapek medium is one to achieve the hichest production rate at a low cost under the optimum condi· dons：pH values within 7 to 9．culture temperature 30℃ and cultivation for 72 h．It is indicated that the suitable pH value for the microbial flocculants to flocculate Kaolin clay suspension is highelthan 7 and the flocculation ratio is up to 94．6％ with the synergy of Ca2 、M and A1¨ ． Keywords：microbial flocculants；flocculants—producing microorganism；flocculation activity；time