H9 N2 亚型AIV HA基因的原核表达及间接ELISA方法的建立.PDF

H N 亚型AIV HA 基因的原核表达及间接 ELISA 方法的建立 9 2 1 1 1 1 1 1 1 ,2 1 郑其升 , 刘华雷 , 张晓勇 , 周 　斌 , 曹瑞兵 ,任雪枫 , 李 　鹏 , 陈溥言 ( 1. 南京农业大学农业部动物疫病诊断与免疫重点开放实验室 , 江苏南京 2 10095 ; 2 . 河北省畜牧兽医站 , 河北保定 07 1000) Prokaryotic Expression and the Establ ishment of a Putative Indirect EL ISA Assay f or the HA Gene of Avian Infl uenza Virus H N subtype 9 2 ZH EN G Qisheng1 , L IU Hualei1 , ZHAN G Xiaoyong1 , ZHOU Bin1 , CA O Ruibing1 , R EN Xuefeng1 , L I Peng1 ,2 , C H EN Puyan1 ( 1. Key L aboratory of A ni m al D isease D i ag nos is an d I m m unology , N anj i ng A g ricul t ural Uni vers ity , N anj i ng 2 10095 , Chi na; ) 2 . A ni m al H us an d ry an d Veteri nary S t at ion of H ebei P rov i nce , B aod i ng 07 1000 , Chi na Abstract : The co mp let e H A gene of A IV H9N2 subt yp e wa s amp lified wit h PCR met ho d u sing a p air of sp ecific p rimer s de signed accor ding to t he relevant nucleotide sequence f ro m GenBank . The PCR p ro duct wa s succe ssively cloned into p MD 18T and p E T32a ( + ) to get a p ro karyotic ( ) exp re ssio n vector p E THA . The t ar get gene wa s succe ssf ully exp re ssed in t he E. col i BL 2 1 D E3 ho st cell in inclu sio n bo dy w