三株丝状真菌分解樟子松凋落物酶活性.PDF

微生物学通报 MAY 20, 2009, 36(5): 635~639 Microbiology © 2009 by Institute of Microbiology, CAS tongbao@ 研究报告 三株丝状真菌分解樟子松凋落物酶活性 1 1 1 2* 宋福强 范晓旭 张星星 宋瑞清 (1. 150080) (2. 150040) 摘 要: 该研究以在樟子松凋落物层中高频出现的3 株丝状真菌Alternaria sp. 、Penicillium sp.和 Pestalotiopsis sp.为供试菌株, 以樟子松新鲜落叶为作用底物, 通过发酵纯培养的方法, 测定了底 物有机物质质量损失及发酵过程中产生的漆酶(Laccase) 、锰过氧化物酶(MnP) 、羧甲基纤维素酶 (CMCase)和滤纸酶(FPA)酶活性变化, 并验证了酶活性与底物降解的关系。结果表明, Alternaria sp. 引起底物总有机物质质量损失最大, 且产生的漆酶、羧甲基纤维素酶和滤纸酶活性都较高; Peni- cillium sp. 产生的锰过氧化物酶活性最高。3 株丝状真菌同属于真菌功能群中的木质纤维素分解者。 关键词: 丝状真菌, 凋落物, 降解率, 木质纤维素酶 Activies of Enzymes During the Litter of Pinus sylvestris var. mongolica Degradation by Three Strains of Mycelial Fungi SONG Fu-Qiang1 FAN Xiao-Xu1 ZHANG Xing-Xing1 SONG Rui-Qing2* (1. Microbiology Key Laboratory of Heilongjiang University, Harbin, Heilongjiang 150080, China) (2. College of Forestry, Northeast Forestry University, Harbin, Heilongjiang 150040, China) Abstract: Three strains of mycelial fungi, Alternaria sp., Penicillium sp. and Pestalotiopsis sp., high- fre- quently isolated from litter of Pinus sylvestris var. mongolica were as the research strain,, the current de- ciduous needles were as the active zymolyte, the method of fermentation was used to assay the mass defect of zymolyte and the activies of enzymes including laccase, manganese peroxidase (MnP), carboxymethyl cellulase (CMCase) and filter paper act