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- 2017-08-04 发布于河南
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克隆羊--多利的克隆过程的介绍(Cloning of sheep - dollys cloning process)
A lamb called Dolly (see photo) sounds around the world. This extraordinary sheep is by the university of Edinburgh Roslin Institute, Edinburghs Roslin Institute) embryologist of Ian Wilmut (Ian Wilmut) led the team from a cell from the udder of an adult sheep cloned.
First, wilmut and his colleagues scraped several membrane cells from the mammary glands of an unnamed Finn Dorset. Normally, this type of cell can maintain its activity in 10% of the fetal serum. Serum is a liquid component of blood, which is rich in cellular nutrients similar to food. Wilmut realized, however, using standard technology of dormant, the cell will reduce them in concentration of 0.5% serum, not only can make the cells they are forget breast cells, and can make them develop as a whole sheep remember genetic instructions.
This is a major breakthrough. Previously, other scientists did not understand the importance of synchronizing cellular genetic material with the egg cells that receive cell genetic material. In the past, in other laboratories, genes were moving too far ahead in the development of egg cells. However, wilmuts cells from Finn Dorsets ewe did not start dividing DNA and translating it into the stuff of sheep. The genes of the resting breast cells are very easy to bind to the egg cells.
The scientists then removed the eggs from the smaller Scottish black sheep and removed their nuclei (the carrier of DNA).
Wiltshire and his team put a cell from the udder of Aries in the black sheep of the egg cell, the nucleus has been removed in a frankenstein (Frankensfein) technique, the electricity pulse makes the membrane of the cells together, not only and two cells instantly becomes one.
Then, using standard artificial reproduction techniques, the resulting egg cells were implanted in a black sheep.
Four months later, the startled lamb was born. It was found to be white, implying that it was not in the same breed as the black-fac
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